当前位置: 首页 > 文章 > 一株间苯二酚降解菌的分离鉴定 东北农业大学学报 2013,44 (8) 53-57
Position: Home > Articles > Isolation and identification of resorcinol degrading strain Journal of Northeast Agricultural University 2013,44 (8) 53-57

一株间苯二酚降解菌的分离鉴定

作  者:
单德鑫;李健;丁丽
单  位:
东北农业大学资源与环境学院
关键词:
煤气废水;酚;降解菌;降解特性;不动杆菌属
摘  要:
对煤气废水活性污泥振荡培养,经驯化、分离筛选出一株能转化间苯二酚的优势降酚菌,命名为JS-4。对不同浓度间苯二酚降解试验表明,培养3 h,菌株JS-4对500、1 000、1 500和2 000 mg.L-1间苯二酚降解率分别为77.62%、55.24%、47.78%和48.25%;降解速率分别达到129.37、184.13、238.90和321.63 mg.L-1。培养24h时,基本可以完全降解上述不同浓度下的间苯二酚(>97.0%),表明JS-4具有较强的降解间苯二酚能力,而且具有较强的抗冲击负荷能力。由生理生化试验和16S鉴定结果可知,菌株JS-4为不动杆菌属。
译  名:
Isolation and identification of resorcinol degrading strain
作  者:
SHAN Dexin,LI Jian,DING Li(School of Resources and Environmental Sciences,Northeast Agricultural University,Harbin 150030,China)
关键词:
coal-gasification wastewater;phenol;degrading bacteria;degradation characteristics;Acinetobacter sp.
摘  要:
The efficient resorcinol degrading bacterial strain JS-4 was acclimated and isolated from coal gasification wastewater activated sludge.Different concentrations of resorcinol degradation tests showed that: For 3 h,strain JS-500,1 000,1 500,and 2 000 mg.L-1 of resorcinol degradation rate were 77.62%,55.24%,47.78% and 48.25%;degradation rates reached 129.37,184.13,238.90 and 321.63 mg.L-1,24 h culture,which could be completely degraded to the different concentration of resorcinol(>97.0%),Strain JS-4 not only had a strong ability to degrade resorcinol,and had some ability to be against shock loading.Combining morphologic characteristics and physiobiochemical characteristics with 16S sequence analysis,strain JS-4 was identified as Acinetobacter sp..

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