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Ⅰ群4型禽腺病毒卵黄抗体被动免疫持续期的研究

作  者:
于洪涛;王昊;刘冬芝
单  位:
哈药集团生物疫苗有限公司
关键词:
卵黄抗体;被动免疫;持续期
摘  要:
本实验取3批实验室试制的卵黄抗体,按1.0m L/只肌肉接种21和42日龄SPF鸡,分别在注射抗体后1、3、5和7d攻毒,观察攻毒保护情况。结果显示,试验鸡接种抗体后1~5d注射I群4型禽腺病毒强毒,攻毒保护率均在90%以上;注射后7d的攻毒保护率为46.7%~50%;各时间点攻毒对照组鸡均90%~100%发病,剖检试验鸡,死亡和发病鸡均呈典型的心包积液及肝脏肿大和坏死的病理变化;健康对照组均100%健活。说明注射抗体后5d抗体仍能产生良好地保护作用,而7d后保护作用大幅减弱。因此,确定I群4型禽腺病毒卵黄抗体被动免疫保护期为5d。
译  名:
Study on the Passive Immunization Duration of Group Ⅰ Type 4 Avian Adenovirus Antibody
作  者:
Yu Hongtao;Wang Hao;Liu Dongzhi;Harbin Pharmaceutical Rroup Bio-vaccine Co.Ltd.;
摘  要:
Three samples of yolk antibody made in laboratory was inoculated to 21-day-old and 42-day-old SPF chickens with 0.5 m L and 1.0 m L, respectively.After the injection of 1, 3, 5, and 7 days, the chickens are challenged with group I type 4 avian adenovirus. The results show that the protection rate of group I type 4 avian adenoviru are both above 90% after 1~5 days injection. The protection rate is only between 70%~76.7% after 7 days injection. The negative control group is 90%~100% death and healthy control group is 100% living. The antibody can produce protection for 5 days of injection, and drops dramaticaly after 7 days of injection. Therefore, the passive immunization duration of the group I type 4 avian adenovirusyolk antibody is 5 days.

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