作 者：
王文绍;程玲;赵肖;李冬冬;索川;马志禹;李俊;安娜;雷治海;苏娟
关键词：
miR-15a;卵泡闭锁;猪卵巢颗粒细胞;转染;BDNF
摘 要：
为探索miR-15a对猪卵泡发育的影响,选取健康大卵泡、中等卵泡和闭锁卵泡,研究不同卵泡中miR-15a的相对表达量以及其是否对卵巢颗粒细胞中有BDNF表达具有调节作用。分别抽取直径3-5 mm、大于5 mm健康卵泡和3-5 mm闭锁猪卵泡,采用q PCR检测发现闭锁卵泡中miR-15a表达显著升高。免疫组化结果显示中等健康卵泡颗粒细胞上BDNF、Tr KB均有表达,而在靠近卵泡腔的凋亡颗粒细胞BDNF免疫阳性反应较弱。分离培养猪卵巢颗粒细胞,过表达miR-15a能显著上调BDNF基因表达水平,Bcl-2基因表达有下降趋势但差异不显著;抑制miR-15a表达能显著抑制BDNF、bcl-2基因表达水平。Western-Blot结果发现,过表达miR-15a显著下调BDNF蛋白水平,而抑制miR-15a表达则能显著上调BDNF蛋白水平。实验结果显示miR-15a可能经由其靶基因BDNF参与猪卵泡的发育和闭锁。
译 名：
MiR-15a inhibits the levels of porcine ovarian granulosa cell's BDNF
作 者：
WANG Wenshao;CHENG Ling;ZHAO Xiao;LI Dongdong;SUO Chuan;MA Zhiyu;LI Jun;AN Na;LEI Zhihai;SU Juan;College of Veterinary Medicine,Nanjing Agriculture University;
关键词：
miR-15a;;atresia;;porcine granulosa cell;;transfection;;BDNF
摘 要：
The aim of our study was to investigate whether miR-15 a has effect on the development of porcine follicular by targeting on BDNF or not. We extracted 3-5 mm healthy,3-5 mm atretic,more than 5 mm healthy follicular and found that the level of miR-15 a in the diameter of 3-5 mm of atretic follicular is more than the healthy follicular by using of q PCR. BDNF and its high-affinity receptor Trk B were stronger expressed than those apoptotic cell which approaching follicular antrum by using immunohistochemistry. After isolating and cultivating porcine follicular cell,overexpression of miR-15 a could significantly upregulate BDNF ' s mRNA and downregulate bcl-2 ' s mRNA without significant difference. Inhibition of miR-15 a could significantly downregulate the expression of BDNF and bcl-2' s mRNA. Western-Blot results showed that overexpression of miR-15 a could obviously downregulate BDNF protein level,while inhibition of miR-15 a can significantly upregulate BDNF level. The results suggest that miR-15 a may be involved in the development and atresia of porcine follicles via targeting BDNF.
