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Position: Home > Articles > Determination of 8 kinds of Glucocorticoids in Health Food by HPLC Food and Nutrition in China 2012,18 (12) 51-53

高效液相色谱法测定保健食品中的8种糖皮质激素

作  者:
高卫东
单  位:
佛山市食品药品检验所
关键词:
高效液相色谱法;保健食品;糖皮质激素
摘  要:
目的:建立了保健食品中8种糖皮质激素的高效液相色谱的测定方法。方法:样品采用甲醇超声提取后,HPLC分离测定。LUBEX Kromasil C18色谱柱(250mm×4.6mm,5μm)分离,流动相为乙腈:水(40:60)、检测波长为241nm、流速为1.0mL/min、柱温为40℃。结果:各糖皮质激素成分在定量范围均称良好的线性关系(r≥0.999),相关系数均大于0.999,平均回收率为96.7%—100.2%,RSD为0.11%—1.71%。结论:本方法准确简便,可应用于保健食品中此8种糖皮质激素的分析。
译  名:
Determination of 8 kinds of Glucocorticoids in Health Food by HPLC
作  者:
GAO Wei-dong(Foshan Institute for Food and Drug Control,Foshan 528000,China)
关键词:
HPLC;health food;glucocorticoid
摘  要:
Objective To establish a method for the simultaneous determination of 8 kinds of glucocorticosteroids in health food by HPLC.Method Samples were extracted with methanol under ultrasonication.The 8 kinds of glucocorticoid were separated on LUBEX Kromasil C18(250mm×4.6mm,5μm)by elution with acetonitrile and water 40:60.The flow rate was 1.0 mL/min,and the detective wavelength was set at 241nm.Result The linear range was 0.027—0.44μg(r>0.999),and the recoveries were 96.7%—100.2% with the relative standard deviations of 0.11%—1.71%.Conclusion The established method is simple feasible,accurate,reproducible and reliable to operate and suitable for the detection of 8 kinds of glucocorticoid in health food.

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