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当前位置: 首页 > 文章 > 光对小麦叶片谷氨酰胺合成酶调节机理初探 华中农业大学学报 2000,19 (2) 102-105
Position: Home > Articles > Preliminary Study on Mechanism of Light Regulated Glutamine Synthetase in Wheat Leaves Journal of Huazhong Agricultural University 2000,19 (2) 102-105

光对小麦叶片谷氨酰胺合成酶调节机理初探

作  者：
王学奎;李合生;刘武定
单  位：
华中农业大学资源环境与农业化学系;华中农业大学农学系
关键词：
小麦;光;抑制剂;谷氨酰胺合成酶
摘  要：
以黄化小麦苗为材料 ,在无钙 (-Ca2 +)和有钙 (+Ca2 +)两种条件下 ,结合不同类型抑制剂以及红光、远红光和白光等不同光质处理 ,初步探讨了光调节小麦叶片谷氨酰胺合成酶 (GS)活性的机理。结果表明 ,黄化小麦叶片经连续 72h光诱导后 ,其GS活性达到正常绿叶水平。有钙培养的小麦幼苗叶片谷氨酰胺合成酶活性高于无钙处理。光对GS活性的调节存在多级水平 ,首先是光对GS的直接激活作用 ,该过程有光敏色素的参与 ,而光敏色素可能是通过钙调系统而起作用的 ,其红光 /远红光逆转效应需要钙的参与 ;其次是光诱导GS的重新合成 ,短期调节主要是从翻译水平上起作用 ,受环己亚胺的抑制 ,长期调节则是在转录水平上发挥作用 ,受放线菌素D的影响
译  名：
Preliminary Study on Mechanism of Light Regulated Glutamine Synthetase in Wheat Leaves
作  者：
Wang Xuekui Li Hesheng Liu Wuding (Huazhong Agricultural University, Wuhan 430070,China)
关键词：
wheat, light, inhibitor, glutamine synthetase
摘  要：
The mechanism of light on regulation of glutamine synthetase (GS) activity of etiolated wheat leaves was preliminarily studied under the treatment with calcium supply and no calcium supply (+Ca 2+ ,-Ca 2+ ) combining with different light and specific inhibitors/antagonist. The results indicated that the GS activity of wheat leaves with calcium in the culture solution was higher than that of treatment with no supply of calcium in the solution, and GS activity of etiolated wheat leaves reached the level of normal green leaves after continuous white light exposure for 72 hours. Multistage regulations of light on GS activity existed in wheat leaves. The first stage regulation, which took place almost immediately after light exposure, was the direct activation of light on GS. Although phytochrome involved in this process, it probably exerted its action via Ca 2+ calmodulin system, and calcium was necessary for the reversible control of the process regulated by red light(R) and far red light(FR); the second stage regulation was light induced de novo synthesis of GS in translation level which was significantly inhibited by cycloheximide, and during a long period light carried out its role in transcription level which was influenced by actinomycin D.

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