Position: Home > Articles > Research on Detection of Extraction of Gentamicin Sulfate by Bovine Serum Albumin-based Foam Separation
Animal Husbandry and Feed Science
2009,30
(6)
30-32
牛血清白蛋白泡沫法提取庆大霉素产物快速检测方法的研究
作 者：
LI Hua-xian
关键词：
foam;gentamicin;concentration;the results;cellulose acetate electrophoresis;criterio
摘 要：
采用醋酸纤维薄膜电泳法分离庆大霉素与牛血清白蛋白,再用紫外分光光度法测定蛋白质泡沫法提取庆大霉素含量的方法。该方法中,选择620nm作定量峰,庆大霉素和氨基黑10B的结合物浓度在1.25～10μg/mL范围内,吸收度与浓度的线性关系良好,相关系数为0.9996,回收率为100.59%。
译 名：
Research on Detection of Extraction of Gentamicin Sulfate by Bovine Serum Albumin-based Foam Separation
作 者：
REN Jian1,,TONG Ying-kai1,WU Zhao-liang2,LI Hua-xiang3 2(1.Tianjin Agricultural University,Tianjin 300384,China;2.Hebei University of Technology,Tianjin 300130,China;3.Jiangnan University,Wuxi 214122,China)
关键词：
Gentamicin;foam extract;rapid detection
摘 要：
In this paper,cellulose acetate electrophoresis and UV spectrophotometry were used to determine Gentamicin in foam extracted by BSA.In UV spectrophotometry method,620 nm was selected as a criterion for quantitative determination.The results indicated that there is a better linear relationship between the absorbance and the concentration of the sample with the condition that combo concentration of gentamicin and amino black 10 B was 1.25 to 10 μg/mL,the correlation coefficient was 0.999 6,and the mean rate of recovery was 100.59%.
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