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Position: Home > Articles > Detection of Citrus psorosis virus by one-step RT-PCR and its annual distribution in different parts of Dweet tangor Plant Protection 2007,33 (3) 42-45

运用一步法RT-PCR检测柑橘鳞皮病毒及其在橘橙不同部位中的全年分布

作者：

吕婵娟;周常勇;周彦;唐科志

单位：

中国农业科学院柑橘研究所;西南大学植物保护学院

关键词：

柑橘鳞皮病毒;一步法RT-PCR;全年分布检测

摘要：

2005年5月到2006年4月逐月采样,运用一步法RT-PCR检测Citrus psorosis virus(CPV)在Dweet橘橙苗木叶片和枝皮中的分布。保存在控温温室中的Dweet橘橙病株中老叶、老皮、嫩叶和嫩皮全年都可以检测出CPV;保存在网室中的Dweet橘橙病株中老叶、老皮全年均能检测到CPV,而夏梢的嫩叶、嫩皮不能稳定地检测出CPV,春、秋梢的嫩叶、嫩皮均可检测到CPV,表明一步法RT-PCR检测CPV最佳取样部位为老叶和老皮。

译名：

Detection of Citrus psorosis virus by one-step RT-PCR and its annual distribution in different parts of Dweet tangor

作者：

Lü Chanjuan1,Zhou Changyong1,2,Zhou Yan1,2,Tang Kezhi2(1.College of Plant Protection,Southwest University,Chongqing 400716, China; 2.Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712,China)

关键词：

Citrus psorosis virus(CPV);one-step RT-PCR;annual distribution

摘要：

From May 2005 to April 2006,annual distribution of Citrus psorosis virus(CPV) on the leaves and shoots of Dweet tangor seedlings was detected by using one-step RT-PCR once a month.CPV was constantly detected in all leaves and barks of the infected plants in greenhouses and in old leaves and barks of the infected plants in screenhouses all the year round.Young leaves and barks of both summer and autumn shoots expect some summer shoots in screenhouses were consistently detected positive for CPV.These results suggested that the most suitable parts of citrus plant for sampling for diagnosis by one-step RT-PCR are old leaves and barks.

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运用一步法RT-PCR检测柑橘鳞皮病毒及其在橘橙不同部位中的全年分布

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