Position: Home > Articles > Research Progress in the Application and Establishment of Bovine Induced Pluripotent Stem Cells
Biotechnology Bulletin
2023,39
(5)
130-141
牛诱导多能干细胞的建立及应用研究进展
作 者：
曾虹;曾睿琳;付伟;吉文汇;兰道亮
单 位：
西南民族大学畜牧兽医学院;青藏高原动物遗传资源保护与利用教育部重点实验室
关键词：
牛诱导多能干细胞;体细胞重编程;培养体系;小分子化合物
摘 要：
诱导性多能干细胞(induced pluripotent stem cells,iPSCs)是指通过导入特定的转录因子将终末分化的体细胞重编程为可以无限增殖更新并具有分化为三胚层多种细胞类型的一类干细胞系.目前对人与小鼠的iPSCs研究已经取得了很多重要成果,但其他动物,如牛等经济型有蹄类家畜iPSCs的研究始终没有突破性的进展.如何将外源转录因子通过重编程载体高效安全地导入体细胞中并持续表达是生产牛诱导多能干细胞(bovine induced pluripotent stem cells,biPSCs)的主要瓶颈.本文就biPSCs建立中重编程系统的选择、诱导因子的选择、小分子化合物的添加等方面进行综述,以期为进一步完善biPSCs及牛胚胎干细胞系的建立提供参考.
译 名：
Research Progress in the Application and Establishment of Bovine Induced Pluripotent Stem Cells
作 者：
ZENG Hong;ZENG Rui-lin;FU Wei;JI Wen-hui;LAN Dao-liang;School of Animal &Veterinary Sciences, Southwest Minzu University;Ministry of Education of Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization;Sichuan Province of Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization;
关键词：
bovine induced pluripotent stem cells;;somatic cell reprogramming;;cultivation system;;small molecular compounds
摘 要：
Induce pluripotent stem cells(iPSCs)refer to a class of stem cell lines that reprogram terminally differentiated somatic cells into infinite proliferation and regeneration and have multiple cell types differentiated into three germ layers by introducing specific transcription factors. At present, many important achievements have been made in the research of iPSCs in humans and mice, but no breakthrough has been made in the research of iPSCs in other animals, such as cattle and other economic ungulates. How to efficiently and safely introduce foreign transcription factors into somatic cells through reprogramming vectors and continuously express them is the main bottleneck in the production of bovine induced pluripotent stem cells. This article reviews the selection of reprogramming system, induction factors and small molecule compounds in the establishment of bovine iPSCs, aiming to provide reference for further improvement of biPSCs and the establishment of bovine embryonic stem cell lines.
