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当前位置: 首页 > 文章 > 脂肪组织细胞膜蛋白卵黄抗体对猪脂肪组织中凋亡相关基因表达的影响 畜牧与兽医 2015,47 (6) 41-45
Position: Home > Articles > Effects of dietary supplementation of egg yolk antibody against adipose plasma membrane on apoptosis-related gene expression in adipose tissue of pigs Animal Husbandry & Veterinary Medicine 2015,47 (6) 41-45

脂肪组织细胞膜蛋白卵黄抗体对猪脂肪组织中凋亡相关基因表达的影响

作  者：
姜锦鹏;赵茹茜;陈杰
单  位：
安徽科技学院动物科学学院;南京农业大学农业部动物生理生化重点开放实验室
关键词：
猪;脂肪组织;脂肪组织细胞膜蛋白卵黄抗体;Bcl-xl;Bax-α;凋亡
摘  要：
探讨脂肪组织细胞膜蛋白卵黄抗体对猪脂肪组织Bcl-xl、Bax-αmRNA表达的影响。将160头体重27.0 kg左右的杜×(长·梅)三元杂交商品猪,随机分成试验组和对照组,每组设4个重复,每个重复20头,公母各半。在试验组基础日粮中添加75 mg/kg脂肪组织细胞膜蛋白卵黄抗体(简称AIg Y)。饲喂104 d后,从每组选出体重相近的12头猪(公母各半)进行屠宰,分离背部皮下脂肪组织、肾周脂肪组织和肠系膜脂肪组织,并称重,同时采集脂肪组织样,采用RT-PCR检测Bcl-xl、Bax-αmRNA表达水平,并测定各部位脂肪组织DNA、RNA浓度,计算RNA、DNA含量以及RNA/DNA比值。结果显示:试验组皮下、肾周、肠系膜脂肪组织DNA含量分别比对照组下降了38.42%(P<0.01)、39.92%(P<0.05)和39.16%,而DNA、RNA浓度以及RNA/DNA比值差异不显著;与对照组相比,试验组脂肪组织Bcl-xl mRNA相对表达丰度下降了18.13%(P<0.05);Bax-αmRNA相对表达丰度升高了13.79%(P<0.05)。提示:日粮中添加脂肪组织细胞膜蛋白卵黄抗体使Bax-α与Bcl-xl基因表达失去平衡,可能诱导猪的脂肪细胞凋亡,上调的Bax-α和下调的Bcl-xl mRNA表达说明AIg Y是通过线粒体途径诱导脂肪细胞凋亡的。
译  名：
Effects of dietary supplementation of egg yolk antibody against adipose plasma membrane on apoptosis-related gene expression in adipose tissue of pigs
作  者：
JIANG Jinpeng;ZHAO Ruqian;CHEN Jie;Institute of Animal Science,Anhui Science and Technology University;Key Laboratory of Animal Physiology and Biochemistry,Ministry of Agriculture,Nanjing Agricultural University;
关键词：
pigs;;adipose tissue;;AIg Y;;Bcl-xl;;Bax-α;;apoptosis
摘  要：
One hundred and sixty crossbred( Duroc-Jersey × Landrace × Meishan) pigs weighing approximately 27. 0 kg at the start of the trail were allocated randomly into treatment and control groups. The treatment group was fed with the diet supplemented with 75 mg / kg yolk antibody against adipose plasma membrane( AIg Y). Following a 104- day trial,the pigs were slaughtered,the weights of fat depots were recorded and three different white adipose tissue depots( subcutaneous fat,perirenal fat and mesenteric fat) were taken for analyzing the DNA content and the abundances of Bcl- xl,Bax- α mRNA by reverse transcription polymerase chain reaction( RT- PCR) using 18 S rRNA as an internal standard. The results showed that the addition of AIg Y reduced DNA content of subcutaneous fat,perirenal fat and mesenteric fat by 38. 42%( P < 0. 01),39. 92%( P < 0. 05) and 39. 16%,respectively. The relative abundance of Bcl- xl mRNA in adipose tissue was decreased by 18. 13%( P < 0. 05),while the relative abundance of Bax- α mRNA in adipose tissue was increased by 13. 79%( P < 0. 05) in treatment group. The results suggest that dietary supplementation of AIg Y causes the imbalance of Bax- α/Bcl- xl expression,which might induce apoptosis in adipose tissue in pigs. The down-expression of Bcl- xl gene and the up- expression of Bax- α gene indicate that apoptosis induced by AIg Y is likely mediated through mitochondrial pathway.

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