中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 不同形态砷对黑藻植物络合素合成的影响 生态学杂志 2012,31 (5) 1233-1240
Position: Home > Articles > Effects of arsenic speciation on the phytochelatins (PCs) synthesis by Hydrilla verticillata Chinese Journal of Ecology 2012,31 (5) 1233-1240

不同形态砷对黑藻植物络合素合成的影响

作  者：
李红梅;王宏镔;王海娟;宋雁辉;钟正燕
单  位：
昆明理工大学环境科学与工程学院
关键词：
重金属;砷胁迫;黑藻;植物络合素
摘  要：
采用反相高效液相色谱(RP-HPLC)法,研究了水培条件下3种形态砷[三价砷As(Ⅲ)、五价砷As(V)和二甲基砷(DMA)]的5个处理浓度(0、0.3、1.0、3.0和5.0mg·L-1)对黑藻(Hydrillaverticillata(L.f.)Royle)砷吸收和植物络合素(PCs)合成的影响。结果表明:黑藻对As表现出明显的吸收和富集效应,低浓度As能促进黑藻生长,对As(Ⅲ)的吸收显著高于As(V)和DMA;As(Ⅲ)和As(V)处理显著诱导了谷胱甘肽(GSH)、PC2和PC4的合成,且与黑藻体内As含量呈显著正相关(P<0.05);PCs对DMA处理不敏感,只有在0.3mg·L-1下能合成PCs;在As(Ⅲ)处理下,GSH和PC2的合成随As(Ⅲ)浓度的增加呈先增加后减少的趋势,而PC4的合成却随As(Ⅲ)浓度的增加而增加;在As(V)处理下,GSH的合成随As(V)浓度的增加呈先增加后减少的趋势,而PC2和PC4的合成则随As(V)浓度的增加而增加;在3种形态As的不同浓度处理中PC3的合成很少,只在0.3mg·L-1下有少量合成。研究结果表明,PCs对As(Ⅲ)和As(V)的胁迫较敏感,可选择性地作为这2种形态砷胁迫下黑藻的生物标记物。
译  名：
Effects of arsenic speciation on the phytochelatins (PCs) synthesis by Hydrilla verticillata
作  者：
LI Hong-mei, WANG Hong-bin, WANG Hai-juan, SONG Yan-hui, ZHONG Zheng-yan (Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650093, China).
关键词：
heavy metal; arsenic stress; Hydrilla verticillata; phytochelatins
摘  要：
A hydroponic experiment was conducted to study the effects of different arsenic species \[As(Ⅲ), As(V), and DMA\] at five concentrations (0, 0.3, 1.0, 3.0, and 5.0 mg·L-1) on the arsenic uptake and phytochelatins (PCs) synthesis by Hydrilla verticillata. The PCs contents were identified by reversed phase-high performance liquid chromatography (RP-HPLC). H. verticillata had an obvious arsenic uptake and accumulation. Low concentrations arsenic promoted the growth of H. verticillata, and the uptake of As(III) by H. verticillata was significantly higher than that of As(V) or DMA. As(Ⅲ) and As(V) promoted the synthesis of GSH, PC2, and PC4 significantly, and the synthesis was significantly positively correlated with the arsenic concentration in H. verticillata (P<0.05). DMA had little effects on the synthesis of PCs, with a detectable synthesis only at 0.3 mg·L-1 of DMA. With increasing concentration of As(Ⅲ), the synthesis of GSH and PC2 decreased after an initial increase, but the synthesis of PC4 increased. W ith the increasing concentration of As(V), the synthesis of GSH increased first and decreased then, while the synthesis of PC2 and PC4 presented an increasing trend. Very few PC3 was synthesized in all treatments except at 0.3 mg·L-1 of the test arsenic species. This study indicated that the synthesis of PCs was sensitive to the stress of As(Ⅲ) and As(V), and the PCs could be used as an biomarker indicating the stress of these two As species on H. verticillata.

相似文章

导出引用
计量
文章访问数: 3
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46