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Position: Home > Articles > EFFECT OF GIBBERELLIC ACID ON THE ACTIVITY OF RIBONUCLEASE FROM BLACK LOCUST SEEDLINGS Forest Research 1990 (1) 34-40

赤霉酸对刺槐实生苗中核糖核酸酶活力的影响

作  者:
陆宪辉
单  位:
南京林业大学
关键词:
刺槐;赤霉酸;核糖核酸酶;~3H-亮氨酸
摘  要:
赤霉酸(纯GA_3)20μg·ml~(-1)处理刺槐(Robinia pseudoacacia L.)实生苗48h后核糖核酸酶(RNase)的活力增加两倍左右,对实生苗的子叶、幼茎和幼根中RNase活力的刺激效应基本相同。5-氟尿嘧啶、亚胺环己酮及高浓度嘌呤霉素可强烈抑制GA_3刺激RNase活力的增加,而放线菌素D则显示出刺激作用。标记RNase的部分提纯,揭露了RNase活力的增加是由于它新合成的缘故。
译  名:
EFFECT OF GIBBERELLIC ACID ON THE ACTIVITY OF RIBONUCLEASE FROM BLACK LOCUST SEEDLINGS
作  者:
Lu Xianhui(Nanjing Forestry University)
关键词:
Black locust; gibberellic acid; ribonuclease; 3H-leucine
摘  要:
There was about two-fold stimulation of RNase activity by the application of GA3 at 20ppm to Black Locust seedlings for 48 hours. Stimulative effect of GA3 on RNase activity to the cotyledons, young stems and young roots of seedlings was basically the same. The increase in activity was inhibited by the administration of 5-fluorouracil,cycloheximide and puromycin but stimulated by the administration of actinomycin D. It seems likely that actinomycin D treatments resulted in maintenance of high levels of enzyme activity by inhibiting the production of substances associated with enzyme decay. Purification of labelled RNase revealed that GA3 enhanced the RNase activity mainly by its fresh synthesis.

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