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壳聚糖固定化超氧化物歧化酶的研究

作  者:
张春艳;孟宪军;宣景宏
单  位:
沈阳农业大学食品学院
关键词:
超氧化物歧化酶;壳聚糖;固定化酶
摘  要:
目的:研究壳聚糖固定化超氧化物歧化酶的酶学性质。方法:分别以不同方法对超氧化物歧化酶进行固定并比较其活力,对固定化方法进行相应的优化,对固定化超氧化物歧化酶进行酶学性质测定。结果:以壳聚糖为载体,戊二醛交联法制备固定化超氧化物歧化酶,优化条件下制备的固定化酶,所得固定化酶活力为330U/g,酶活回收率为58.33%,热稳定性和酸稳定性较游离酶有很大的提高,且具有良好的贮存稳定性,固定化酶可实现反复使用,提高了利用率。结论:壳聚糖-戊二醛交联法可用于制备性能较优的固定化超氧化物歧化酶。
译  名:
Study on Supeoxide Dismutase Immobilized with Chitosan
作  者:
ZHANG Chun-yan,MENG Xian-jun,XUAN Jing-hong (College of Food Science, Shenyang Agricultural University, Shenyang 110161, China)
关键词:
superoxide dismutase;chitosan;immobilized enzyme
摘  要:
Objective: To study the enzyme properties of superoxide dismutase immobilized with chitosan. Methods: Immobilized superoxide dismutase were prepared by different processes. The methods were optimized by comparing their activities. Results: Superoxide dismutase on chitosan with glutaraldehyde as crosslinking agent showed the highest activities and recovery rates.The activity of superoxide dismutase powder immobilized under optimum conditions is 330 U/g,with the recovery rate above 58.33%.The temperature stability and the pH stability of the immobilized enzyme are much better than the free enzyme.And the immobilized enzyme also shows a good storage stability. The immobilized enzyme may be applied repeatedly. It can improve untilization rate. Conclusion: The immobilized superoxide dismutase can be prepared by crosslinking glutaraldehyde on chitosan, and it possesses good properties.

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