Position: Home > Articles > Establishment of Promoter Trapping System in Torenia fournieri Linden
Northern Horticulture
2008
(5)
181-184
蓝猪耳启动子捕获系统的建立
作 者：
龙海涛;李洪清;李玲
单 位：
华南师范大学生命科学学院
关键词：
启动子捕获;T-DNA侧翼;GUS报告基因;蓝猪耳
摘 要：
为了分离基因启动子,构建了陷阱植物表达载体pHAHCA,用农杆菌介导的无启动子GUS方法转化蓝猪耳,经抗性筛选和PCR检测,获得82株转基因植株,转化率达12.5%。12株GUS染色呈阳性,其中10株GUS在叶脉表达,2株在茎和叶脉表达。对转基因植株进行盐胁迫处理,有1株根部出现GUS染色蓝色斑点。通过TAIL-PCR扩增和测序,获得5个T-DNA侧翼序列。
译 名：
Establishment of Promoter Trapping System in Torenia fournieri Linden
作 者：
LONG Hai-tao,LI Hong-qing,LI Ling(College of Life Science,South China Normal University,Guangzhou,Guangdong 510631,China)
关键词：
Promoter trapping;Genetic transformation;GUS reporter gene;Torenia fournieri Linden
摘 要：
Based on the optimization of genetic transformation of Torenia fournieri Linden mediated by Agrobacterium tumefaciens,a binary vector,designated as pHAHCA,was constructed for promoter trapping,and was introduced into Torenia to establish an efficient promoter trapping system.82 hygromycin-resistant plants were confirmed to be positive transformants by PCR.The transformation frequency was up to 12.5% overall.GUS histochemical staining of the 82 transgenic plants showed that 12 of them were stained.10 out of the 12 transformants plants were stained only in veins of plants,and the other 2 transgenic plants were stained both in veins and stems.Under salt stress,only one plant showed GUS activity in roots.The flanking sequences of T-DNA inserting sites in 5 transgenic Torenia showed GUS activity was amplified by TAIL-PCR.
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