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当前位置: 首页 > 文章 > 汞毒性对草木樨(Melilotus officinalis)幼苗基因型的差异性抑制研究 农业环境科学学报 2010,29 (01) 66-71
Position: Home > Articles > Differential Inhabitation of Mercury Toxicity to Seedlings of Sweet Clover(Melilotus officinalis) Genotypes Journal of Agro-Environment Science 2010,29 (01) 66-71

汞毒性对草木樨(Melilotus officinalis)幼苗基因型的差异性抑制研究

作  者：
王钰婧;李伟;张艳红;李翠兰;张秋华;段毅豪
单  位：
山西大学生命科学与技术学院;山西大学环境与资源学院
关键词：
草木樨;等位酶;基因型;汞毒性;上胚轴;下胚轴
摘  要：
采用水平淀粉凝胶电泳技术对草木樨(Melilotus officinalis)的磷酸葡萄糖异构酶(Pgi)和苹果酸酶(Me)进行了基因频率分析。所研究的草木樨种群具有较高的多态性(A=3.0,Ho=0.346～0.401),将草木樨种子暴露于HgCl2溶液(30mg·L-1),萌发后测量各幼苗的上、下胚轴长度以及测定其基因型,判定各基因型与上、下胚轴长度的关系。ANOVA分析表明,对照组中Me-AA基因型幼苗具有下胚轴生长优势,Me-AA基因型个体的下胚轴长度(35.20mm±4.63mm)显著大于以下各基因型个体的下胚轴长度:Me-AB(22.84mm±1.34mm),Me-BB(22.82mm±0.68mm)和Me-BC(20.00mm±1.21mm)。HgCl2急性暴露对Me各基因型下胚轴的抑制作用呈现均一性。在Pgi基因座,草木樨对照组中各基因型的生长不存在差异,但在HgCl2急性暴露下,Pgi-AC基因型幼苗的下胚轴长度(19.75mm±0.73mm)显著大于Pgi-BB基因型的下胚轴长度(14.67mm±1.04mm),表明Pgi-AC基因型个体对HgCl2的耐受性更强。等位酶分析技术可用来确定植物的种群遗传结构对生长或重金属的耐受性关系,这种关系的确立对遗传育种、生态修复具有潜在的应用价值。
译  名：
Differential Inhabitation of Mercury Toxicity to Seedlings of Sweet Clover(Melilotus officinalis) Genotypes
作  者：
WANG Yu-jing1,LI Wei1,ZHANG Yan-hong1,LI Cui-lan2,ZHANG Qiu-hua1,DUAN Yi-hao1(1.School of Environmental Science and Resources,Shanxi University,Taiyuan 030006,China;2.School of Biological Science and Technology,Shanxi University,Taiyuan 030006,China)
关键词：
Melilotus adans;allozyme;genotype;mercury toxicity;epicotyls;hypocotyls
摘  要：
The horizontal starch gel electrophoresis was used to examine the polymorphic allozymes of phosphoglucose isomerase(Pgi)and malic enzyme(Me)in sweet clover(Melilotus officinalis).The samples showed relatively high polymorphism at the loc(iA=3.0,Ho=0.346～ 0.401),good for allozyme analysis.The seeds of sweet clover were acutely exposed to HgCl(230 mg·L-1)for germination.The allozyme genotypes of each seedling were determined and the length of epicotyls and hypocotyls were measured to explore the relationship relationships between the alloyzme genotypes and tolerance to HgCl2.ANOVA showed the results as follows.At Me locus the seedlings of Me-AA genotype showed growth vigor,as demonstrated by longer hypocotyls of Me-AA(35.20 mm±4.63 mm)than that of Me-AB(22.84 mm±1.34 mm),Me-BB(22.82 mm±0.68mm)and Me-BC(20.00 mm±1.21mm).The exposure to HgCl2 resulted in uniform inhibition among all genotypes at Me.At Pgi locus the ANOVA results exhibited no difference in both epicotyl and hypocotyl growth among the genotypes in control group.However BB(14.67 mm±1.04 mm),suggesting that Pgi-AC genotype is more resistant to HgCl2.These results demonstrates the usefulness of alloyme analysis in determining the relationships of plant population genetic structure to growth and/or resistance to mercury,which is potentially applicable to genetic breeding and breeding,as well as ecological rehabilitation.It is suggested to include genetic diversity indices like allozyme polymorphism into the assessment of environmental quality.

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