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当前位置: 首页 > 文章 > 107杨次生木质部PAL基因的RT-PCR扩增及其鉴定 林业科学 2004,40 (4) 192-196
Position: Home > Articles > Cloning and Identification of PAL Gene Amplified by RT-PCR from Populus×euramericana cv.“74/76” Second Xylem mRNA Scientia Silvae Sinicae 2004,40 (4) 192-196

107杨次生木质部PAL基因的RT-PCR扩增及其鉴定

作  者：
薛永常;李金花;卢孟柱;张绮纹
单  位：
大连轻工业学院生物与食品学院;中国林业科学研究院林业研究所
关键词：
欧美杨107;苯丙氨酸解氨酶(PAL);RT-PCR;载体构建
摘  要：
A fragment of PAL (phenylalanine ammonia_lyase) gene was amplified by RT_PCR from poplar (Populus×euramericana cv. “74/76”) developing second xylem mRNA. It was cloned into pGEM-T Easy vector and identified by restriction enzyme, PCR amplification and sequencing. The sequence of the amplified DNA fragment was 565 base pairs. Alignment with the P. kitakamiensis PAL cDNA sequence retrieved from EMBL nucleotide acid database (accession number D30656) showed that the first 400 base pairs in both sequences were almost identical. Therefore the fragment was part of PAL gene. And both of sense and anti-sense expressional vectors were constructed.
译  名：
Cloning and Identification of PAL Gene Amplified by RT-PCR from Populus×euramericana cv.“74/76” Second Xylem mRNA
作  者：
Xue Yongchang (College of Bio. & Food Tech., Dalian Institute of Light Industry Dalian116034) Li Jinhua Lu Mengzhu Zhang Qiwen(Research Institute of Forestry,CAF Beijing100091)
关键词：
Populus×euramericana cv.“74/76", Phenylalanine ammonia-lyase(PAL), RT-PCR, Vector construction
摘  要：
A fragment of PAL (phenylalanine ammonia_lyase) gene was amplified by RT_PCR from poplar (Populus×euramericana cv. “74/76”) developing second xylem mRNA. It was cloned into pGEM-T Easy vector and identified by restriction enzyme, PCR amplification and sequencing. The sequence of the amplified DNA fragment was 565 base pairs. Alignment with the P. kitakamiensis PAL cDNA sequence retrieved from EMBL nucleotide acid database (accession number D30656) showed that the first 400 base pairs in both sequences were almost identical. Therefore the fragment was part of PAL gene. And both of sense and anti-sense expressional vectors were constructed.

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