中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 6个中国小型猪种SLA-DQB基因外显子2多态性分析 中国畜牧杂志 2017,53 (8) 26-29
Position: Home > Articles > Genetic Polymorphism of Exon 2 of SLA-DQB Genes in Six Miniature Pig Breeds Chinese Journal of Animal Science 2017,53 (8) 26-29

6个中国小型猪种SLA-DQB基因外显子2多态性分析

作  者：
朱晓萍;杨雪梅;李华;于辉;李学伟
单  位：
佛山科学技术学院
关键词：
小型猪;SLA-DQB;PCR-RLFP;多态性
摘  要：
采用PCR-RFLP法对甘孜藏猪、合作猪、滇南小耳猪、荷包猪、五指山猪和巴马小型猪SLA-DQB基因外显子2多态性进行分析。结果表明:经RsaⅠ酶切检测到5种等位基因,14种RFLP带型,遗传杂合度(He)在0.538~0.729,多态信息含量(PIC)在0.488~0.728,该位点表现为高度多态;χ~2适合性检验显示,甘孜藏猪、巴马小型猪和五指山猪3个群体处于Hardy-Weinberg平衡状态。经HinfⅠ酶切检测到3种等位基因,6种RFLP带型,He在0.315~0.524,PIC在0.291~0.461,在该位点表现为中度多态。χ~2适合性检验显示,甘孜藏猪、滇南小耳猪、巴马小型猪、荷包猪4个群体处于Hardy-Weinberg平衡状态。因此,6个小型猪种的SLA-DQB基因外显子2多态性丰富且存在明显差异。
译  名：
Genetic Polymorphism of Exon 2 of SLA-DQB Genes in Six Miniature Pig Breeds
作  者：
ZHU Xiao-ping;YANG Xue-mei;LI Hua;YU Hui;LI Xue-wei;Foshan University;Sichuan Academy of Animal Sciences;Sichuan Agricultural University;
关键词：
Miniature pigs;;SLA-DQB gene;;PCR-RLFP;;Polymorphism
摘  要：
The exon 2 polymorphism of SLA-DQB gene in Ganzhi Zang pigs(Tibet pigs), Hezuo pigs, Diannan Smalleared pigs, Hebao pigs, Wuzhishan pigs and Bama miniature pigs were studied using PCR-RFLP technique. The results showed that there were five alleles and fourteen RFLP genotypes with restriction enzyme RsaⅠ digestion, polymorphism of the DQB exon 2 was abundant. Its genetic heterozygosity was 0.538~0.729, and polymorphic information content was 0.488~0.728. The results of χ~2 showed that five alleles of Rsa I locus in Ganzhi Zang pigs,Bama pigs and Wuzhishan pigs fit with Hardy-Weinberg equilibrium. There were three alleles and six RFLP with restriction enzyme Hinf I digestion. Its genetic heterozygosity was 0.315~0.524, and polymorphic information content was 0.291~0.461.The locus was moderately polymorphic. The results of χ~2 showed that 3 alleles of Hinf I locus in Ganzhi Zang pigs、Diannan pigs、Bama pigs and Hebao pigs were fit with Hardy-Weinberg equilibrium. The results suggested that there was significant difference in six miniature pigs.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46