Distribution of diffused neuroendorine system cells in the goat corpus luteum

CHEN Shu-lin1, JIANG Tian-yuan1, LEI Zhi-hai2, LIN Lei1ZENG Ming-hua1, LI Wen-xian3, FAN Guang-li1(1.College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 2.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; 3.Henan Engineering Technology Research Center for Dairy Products, Zhengzhou 450008, China)

diffused neuroendorine system; corpus luteum; streptaridin-peroxidase; dairy goat

Existence of diffused neuroendocrine system(DNES)cells in the corpus luteum of dairy goat has been determined by an immunohistochemistry streptavidin-peroxidase(SP)method. Using the SP method, the corpus luteum of 12-18-month-old dairy goats during pregnancy were studied and the following cell types were identified throughout the corpus luteum: oxytocin, neuron specific enolase, S-100 protein, synaptophysin, 5-hydroxytryptamine immunoreactive cells. The cells existed as individual or clustered. They were round, oval or triangle in shape. Some cells have obvious neuritis. The immunoreactive cells in one section in descending order were oxytocin, synaptophysin, S-100 protein, neuron specific enolase and 5-hydroxytryptamine. The existence of DNES cells was inferred from the presence of neuron specific enolase, S-100 protein, synaptophysin and 5-hydroxytryptamine which are the broad-spectrum markers for DNES.