关键词：
NTG;LiCl;复合诱变;链霉菌702;新农抗702
摘 要：
为筛选出产新农抗702的高产链霉菌702突变株。分别以链霉菌702菌株为试验材料和以庆大霉素为敏感抗生素,NTG-LiCl复合诱变链霉菌702菌株,获得抗庆大霉素突变株。NTG处理90min对菌株的致死率可达71.56%,抗药性突变率高达19.46%,获得的抗药性突变株经过摇瓶初筛和复筛,获得高产突变株13-18-52菌株,产抗新农抗702的摇瓶发酵单位达到1946μg/mL,比出发菌株发酵单位1416μg/mL提高了37.43%。采用抗药性致死突变标志的NTG-LiCl复合诱变筛选模型可以获得产新农抗702的链霉菌702高产菌株。
译 名：
Mutation Screening of Streptomyces 702 by NTG and LiCl
作 者：
TU Xuan,ZHOU Yun,YE Rong-hua,TU Guo-quan(Biological Science & Engineering College,Jiangxi Agricultural University,Nanchang 330045,China)
关键词：
NTG;LiCl;composite mutation;Streptomyces 702;new ag-antibiotics 702
摘 要：
Purpose: To screen out a Streptomyces 702 strain of high yield of New Ag-antibiotics 702.Methods: Gentamycin-resistance mutational labelling selection model with Streptomyces 702 strain ws used as the experimental material and Gentamycin as sensitive antibiotic to screening high-yield mutants.The Streptomyces 702 spores was treated by NTG-LiCl composite mutation.Results: The results showed that the gentamycin-resistance mutational frequency of the streptomyces 702 treated by NTG-LiCl with 90 min utes was up to 19.46% and the lethal rate was 71.56% .High New Ag-antibiotics 702-producing mutant 13-18-52 was isolated in the screening experiments,and its yield of New Ag-antibiotics 702 reached 1946μg/mL.which was 37.43% higher than that produced by its parent strain.Conclusion: The Streptomyces 702 strain of high New Ag-antibiotics 702 could be obtained by NTG-LiCl composite mutation and resistance-chemical mutational labelling selection model.
