中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 生物质炭固定化融合菌株F14方法的研究及其对芘的去除 农业环境科学学报 2018 (03) 464-470
Position: Home > Articles > Degradation of pyrene by immobilized cells of fusant bacterial strain F14 using biochar Journal of Agro-Environment Science 2018 (03) 464-470

生物质炭固定化融合菌株F14方法的研究及其对芘的去除

作  者：
张松;侯彬;纪婷婷;刘阳;黄飞;秦梓菲;卢静
单  位：
中北大学环境与安全工程学院;华南农业大学资源环境学院
关键词：
融合菌株;固定化微生物;生物质炭;多环芳烃
摘  要：
F14是一株用原生质体融合技术构建的能够降解多环芳烃(PAHs)的融合菌株,利用生物质炭固定化F14制备固定化微生物小球来提高该菌株对芘的去除能力。实验在确定聚乙烯醇(PVA)和海藻酸钠(SA)最佳组合比例的基础上,研究了不同温度下(300、500℃和700℃)制备的生物质炭形成的固定化小球的性能以及对芘的去除效果。结果表明:制备的固定化小球的直径约为3mm,对固定化小球成球效果、传质性能和机械性能的分析表明,载体PVA和SA的最佳组合比例为8%和1%;对不同温度下制备的生物质炭的固定化小球的传质性能和扫描电镜图进行分析,表明700℃制备的生物质炭的固定化小球性能较好。与游离菌相比,固定化小球对芘的去除能力更高且速率更快,对初始浓度为50 mg·L~(-1)的芘10 d内去除率高达94.91%,比游离菌对芘的去除率高了近58%,同时固定化小球可以重复使用高达9次。
译  名：
Degradation of pyrene by immobilized cells of fusant bacterial strain F14 using biochar
作  者：
ZHANG Song;HOU Bin;JI Ting-ting;LIU Yang;HUANG Fei;QIN Zi-fei;LU Jing;School of Environment and Safety Engineering, North University of China;The College of Natural Resources and Environment, South China Agricultural University;
关键词：
fusant bacterial strain;;immobilized microorganism;;biochar;;PAHs
摘  要：
F14 is a fusant strain constructed by protoplast fusion and is able to degrade polycyclic aromatic hydrocarbons(PAHs). To improve the pyrene removal rate, F14 was immobilized using biochar. The removal of pyrene by immobilized beads with added biochar produced at different temperatures(300, 500 ℃, and 700 ℃)was investigated, based on a study of the optimum combination ratio of polyvinyl alcohol(PVA)and sodium alginate(SA). The results showed that the diameter of the immobilized beads was about 3 mm, and an analysis of the effects, mass transfer, and mechanical properties of the immobilized beads showed that the best ratios of PVA and SA were 8% and 1%,respectively. Further analysis of mass transfer and scanning electron microscopy of the immobilized beads made by different biochars showed that the immobilized beads made from 700 ℃ biochar had better performance. Immobilized cells presented a higher and faster removal ability than free cells for pyrene, and 94.91% of pyrene could be removed within 10 d from an initial concentration of 50 mg·L~(-1). The percentage removed by the immobilized cells was 58% higher than that removed by free cells. In addition, the immobilized beads could be reused up to 9 times.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46