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当前位置: 首页 > 文章 > 水稻脆秆褐穗突变体fb1的鉴定与基因定位 核农学报 2017 (12) 2298-2305
Position: Home > Articles > Identification and Gene Fine Mapping of Fragile-plant and Brown-panicle Mutant fb1 in Rice Journal of Nuclear Agricultural Sciences 2017 (12) 2298-2305

水稻脆秆褐穗突变体fb1的鉴定与基因定位

作  者：
王晓雯;唐彦强;廖红香;蒋钰东;杨正林;桑贤春
关键词：
水稻;褐穗;脆秆;纤维素;基因定位
摘  要：
为鉴定新的脆杆突变体,从籼型水稻恢复系缙恢10号的甲基磺酸乙酯(EMS)诱变体库中鉴定了一个全生育期植株变脆且开花后稻穗呈褐色的突变体,暂命名为脆秆褐穗突变体fb1,对其进行了机械强度、光合色素含量、木质素和纤维素含量等测定,并开展了遗传分析和基因定位等研究。结果表明,与野生型相比,fb1茎秆和叶片的最大载荷分别下降了43.3%和63.1%,茎秆的纤维素和木质素含量分别为野生型的76.5%和66.6%,叶片的纤维素和木质素含量则分别为野生型的91.0%和95.5%,均显著降低。开花前,fb1的穗部性状与野生型相比无变化,开花后颖壳逐渐褐化,成熟后呈灰褐色。fb1的叶绿素a、叶绿素b和类胡萝卜素含量分别为野生型的74.8%、90.5%和85.3%,均呈显著或极显著下降。遗传分析表明,该性状受一对隐性核基因调控,基于西农1A/fb1的F2群体,利用SSR等分子标记最终将调控基因FB1精细定位在第1染色体SSR标记RM1268和RM11669之间42 kb的物理范围内,包含7个注释基因。该研究结果不仅为FB1基因的图位克隆奠定了基础,也为植物细胞壁发育分子机理研究和新能源水稻育种提供了材料。
译  名：
Identification and Gene Fine Mapping of Fragile-plant and Brown-panicle Mutant fb1 in Rice
作  者：
WANG Xiaowen;TANG Yanqiang;LIAO Hongxiang;JIANG Yudong;YANG Zhenglin;SANG Xianchun;Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute of Southwest University;Institute of Rice and Sorghum,Sichuan Academy of Agricultural Science;
单  位：
Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute of Southwest University%Institute of Rice and Sorghum,Sichuan Academy of Agricultural Science
关键词：
rice;;brown panicle;;fragile curm;;cellulose;;gene mapping
摘  要：
An EMS-induced library of indica rice restorer line Jinhui10 was screened and a mutant displaying fragile plant with brown panicle was discovered,temporally termed as fb1. The characteristics of its mechanical strength,photosynthetic pigment content,lignin and cellulose content were detected,meanwhile,genetic analysis and gene mapping were performed in this study. The mechanical strengths of fb1 decreased 43. 3% and 63. 1% in stems and leaves respectively than those of the wild type. Correspondingly,the contents of cellulose and lignin in fb1 stem occupied76. 5% and 66. 6% of the wild type's,and in fb1 leaves was 91% and 95. 5%,respectively of those of the wild type.All these differences were statistically significant. However, panicle-related traits of fb1 displayed no significant difference when compared to those of the wild type before blossom. After blossoming,the glume in fb1 exhibited gradually brown and even dust-color. By analysis of photosynthetic pigment content,the contents of chlorophyll a,chlorophyll b and carotenoids in fb1 were only 74. 8%,90. 5% and 85. 3% of that of the wild type respectively,which displayed statiscally significantl declines. Genetic analysis indicated that the mutational characteristics were regulated by one recessive nuclear gene. FB1 was finally mapped on chromosome 1 between SSR markers RM1268 and RM11669 with 42 kb physical distance,in which contained seven annotated genes. These results not only provide a good foundation for FB1 cloning by a map-based strategy,but also supply an excellent material for studying molecular mechanism of cell wall development and breeding new environmental protection energy rice.

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