中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 迟钝爱德华氏菌外膜蛋白基因工程表达产物的免疫原性 华中农业大学学报 2016,35 (5) 97-104
Position: Home > Articles > Immunogenicity of outer membrane protein expressed by gene engineering of Edwardsiella tarda Journal of Huazhong Agricultural University 2016,35 (5) 97-104

迟钝爱德华氏菌外膜蛋白基因工程表达产物的免疫原性

作  者：
段丽华;冯建军;郭松林;陈义航;阮彩章
单  位：
集美大学水产学院/鳗鲡现代产业技术教育部工程研究中心
关键词：
日本鳗鲡;迟钝爱德华氏菌;外膜蛋白;免疫原性;相对免疫保护率
摘  要：
构建迟钝爱德华氏菌外膜蛋白重组表达载体后,表达纯化获得分子质量约53ku的外膜蛋白。将100尾日本鳗鲡均分为2组,分别腹腔注射牛血清白蛋白(BSA,0.5mg/mL)和迟钝爱德华氏菌外膜蛋白(Omp,0.5mg/mL)0.2mL/尾。于免疫后第7、14和28天采集鳗鲡血液、粘液、肝脏和肾脏,测定各时间点的补体活性、溶菌酶活性、全血细胞转化水平和血清抗体效价。结果发现,外膜蛋白组鳗鲡的补体活性于免疫后第7天极显著高于对照组;其抗体效价于第14天和第28天显著或极显著高于对照组。免疫后第28天以迟钝爱德华氏菌、嗜水气单胞菌和创伤弧菌(1.0×107 cfu)腹腔注射感染鳗鲡。结果发现,外膜蛋白组鳗鲡对迟钝爱德华氏菌、嗜水气单胞菌和创伤弧菌的相对免疫保护率分别为71.4%、50.0%和12.5%。表明鳗鲡注射迟钝爱德华氏菌基因工程表达外膜后显著提高了其对迟钝爱德华氏菌感染的抵抗力,对嗜水气单胞菌的感染也有明显的交叉保护效果,但对创伤弧菌的交叉保护效果较弱。
译  名：
Immunogenicity of outer membrane protein expressed by gene engineering of Edwardsiella tarda
作  者：
DUAN Lihua;FENG Jianjun;GUO Songlin;CHEN Yihang;RUAN Caizhang;Fisheries College of Jimei University/Engineering Research Center of the Modern Industry Technology for Eel,Ministry of Education;
关键词：
Anguilla japonica;;Edwardsiella tarda;;outer membrane protein;;immunogenicity;;relative percent survival(RPS)
摘  要：
The expressed outer membrane protein(Omp,about 52ku)of Edwardsiella tarda was purified after the recombinant expression vector was constructed,transferred into E.coli BL21 and induced.A total of 100 healthy Japanese eels(Anguilla japonica)was divided into 2groups and intraperitoneally injected(i.p)with bovine serum albumin(BSA,0.5mg/mL)and the outer membrane protein(Omp,0.5 mg/mL),respectively.On 7,14 and 28dpost-vaccination,the blood,skin mucus,liver and kidney of eels were collected to evaluate the lysozyme and complement activities,stimulation index and specific antibody titers.The complement activities in the Omp group were extremely significantly(P<0.01)higher than that of the BSA group on 7d.The specific antibody titers in the Omp group were significantly(P<0.05 or P<0.01)higher than that of the BSA group on 14 dand 28d.The lysozyme activities and stimulation indexes showed no significant difference(P>0.05)between two groups.The relative percent survival(RPS)of Omp were 71.4%,50.0% and 12.5% respectively after challenged by E.tarda,A.hydrophila or V.vulnificus.These results suggested that the recombinant expressed Omp could significantly improve the immune function and resistance of A.japonicaagainst E.tarda.Italsohadsignificant cross protection against A.hydrophila,but had weak cross protection against V.vulnificus.

相似文章

导出引用
计量
文章访问数: 3
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46