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Position: Home > Articles > APPLICATION OF PCR RFLPs TESTING METHOD TO CONSTRUCTION OF PIETRAIN PIG POPULATION Acta Agriculturae Shanghai 2001,17 (3) 34-37

PCR-RFLPs检测法在无氟烷隐性基因皮特兰猪建群中的应用

作  者:
谈永松;张似青;张忠明;何玉英;刘红林;王林云
单  位:
上海市农业科学院畜牧兽医研究所;南京农业大学动物科技学院
关键词:
猪;皮特兰猪;PCRRFLPs;氟烷基因
摘  要:
采用PCR RFLPs法对上海市农业科学院畜牧兽医研究所试验猪场的皮特兰猪进行氟烷基因检测 ,结果表明 ,在 4 0头皮特兰猪中 ,检测到具有NN氟烷基因型的猪 1头 ,具有Nn基因型的 9头 ,其余的为nn型 ,试验结果提示 ,采用此法对皮特兰猪的氟烷基因进行检测不但可行 ,而且还可依据此结果进行无氟烷隐性基因皮特兰猪群的选育
译  名:
APPLICATION OF PCR RFLPs TESTING METHOD TO CONSTRUCTION OF PIETRAIN PIG POPULATION
作  者:
WITH NON HALOTHANE ALLOGENE Tan Yongsong Zhang Siqing Zhang Zhongming (Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106) He Yuying Liu Honglin Wang Linyun (Animal Science and Technology
关键词:
Pig; Pietrain pig; PCR RFLPs; Halothane gene
摘  要:
The halothane gene of Pietrain pigs in the experimental pig farm of SAAS was tested by PCR RFLPs testing method. The results showed that among 40 test pigs there were 9 pigs with Nn genotype, 1 pig with NN genotype, and 30 pigs with nn genotype, and so implied that this method is available to test the halothane genotype of Pietrain pigs and according to the testing results,the selection and breeding of Pietrain pig population with non halothane allogene can be implemented.

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