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当前位置: 首页 > 文章 > ELISA法与LC-MS/MS法测定动物组织中克仑特罗残留 肉类研究 2011 (11) 38-42
Position: Home > Articles > Enzyme-linked Immunosorbent Assay (ELISA) and LC-MS/MS for Determination of Clenbuterol Residue in Animal Tissue MEAT RESEARCH 2011 (11) 38-42

ELISA法与LC-MS/MS法测定动物组织中克仑特罗残留

作  者：
万宇平;刘宁;李金超;汪善良;聂雯莹
单  位：
临沂新程金锣肉制品集团有限公司;河北省兽药监察所;北京勤邦生物技术有限公司
关键词：
克仑特罗;动物组织;酶联免疫法(ELISA);液相色谱-串联质谱法(LC-MS/MS)
摘  要：
目的:比较酶联免疫法(ELISA)和液相色谱-串联质谱法(LC-MS/MS)测定动物组织中克仑特罗残留量的准确度、精密度及检测限。方法:样品经过处理后,分别采用ELISA法和LC-MS/MS法进行测定;用ELISA法对组织样品进行初筛,测出阳性样品利用LC-MS/MS法进行确证。结果:应用ELISA法测定样品猪肉样品中克仑特罗的回收率为67.0%～99.6%,批内变异系数3.4%～8.7%,批间变异系数6.1%～9.6%,灵敏度为0.025μg/L,最低检测限0.025μg/kg;LC-MS/MS检测方法回收率88.62%～111.43%,批内变异系数4.4%～7.4%,最低检测限0.5μg/kg;用ELISA法对50份猪肉和50份猪肝样品进行检测,筛选出3个阳性样品,经LC-MS/MS确证为阳性,两种方法检测结果一致。结论:ELISA法灵敏度和准确度较高,样品处理方法简单,成本低,适合组织中克仑特罗残留大规模筛查;LC-MS/MS准确度高,适合于阳性样品精确定量。
译  名：
Enzyme-linked Immunosorbent Assay (ELISA) and LC-MS/MS for Determination of Clenbuterol Residue in Animal Tissue
作  者：
WAN Yu-ping 1,LIU Ning 2,LI Jin-chao 3,WANG Shan-liang 1,NIE Wen-ying 1(1.Beijing Kwinbon Biotechnology Co.Ltd.,Beijing 102206,China;2.Linyi Xincheng Jinluo Meat Products Co.Ltd.,Linyi 276036,China;3.Hebei Institute of Veterinary Drugs Control,Shijiazhuang 050051,China)
关键词：
clenbuterol;animal tissue;enzyme-linked immunosorbent assay(ELISA);liquid chromatography tandem mass spectrometry(LC-MS/MS)
摘  要：
Objectives: To compare the precision,accuracy and limit of detection of enzyme-linked immunosorbent assay(ELISA)and liquid chromatography tandem mass spectrometry(LC-MS/MS) in determining clenbuterol residue in animal tissue.Methods: Samples were prepared and then determined by ELISA and LC-MS/MS,respectively.LC-MS/MS was used to confirm positive samples from ELISA analysis.Results: The ELISA recoveries across three spike levels,intra-batch and inter-batch coefficients of variation,sensitivity and limit of detection were 67.0%-99.6%,3.4%-8.7%,6.1%-9.6%,0.025μg/L and 0.025μg/kg,respectively.For LC-MS/MS,the recoveries across three spike levels were 88.62%-111.43%,the intra-batch coefficients of variation 4.4%-7.4%,and the limit of detection 0.5μ g/kg.Three positive samples were screened out of 50 pork samples and 50 pig,s liver samples using ELISA,which was consistent with the results from LC-MS/MS analysis.Conclusion: ELISA is sensitive and accuracy and needs easy sample preparation and low cost,thus being suitable for large-scale screening of clenbuterol residue in animal tissue.In contrast,LC-MS/MS has higher accuracy and can therefore be used to quantify positive samples.

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