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当前位置: 首页 > 文章 > 石河子地区棉花黄萎病菌培养特性和致病力分化研究 新疆农业科学 2011,48 (3) 522-527
Position: Home > Articles > Study on the Culture Characteristic and Pathogenic Differentiation of Veticillium dahliae from Cotton in Shihhotze Xinjiang Agricultural Sciences 2011,48 (3) 522-527

石河子地区棉花黄萎病菌培养特性和致病力分化研究

作  者：
韩宏伟;任毓忠;高峰;刘培源;吉丽丽;李春;李国英
单  位：
石河子大学绿洲农作物病害防控重点实验室
关键词：
棉花;黄萎病菌;培养特性;致病力分化;落叶症状;石河子地区
摘  要：
【目的】明确当前石河子地区棉花黄萎病菌致病力分化的情况,从病原角度入手搞清病害明显加重的原因。【方法】采用鉴别寄主法和分子生物学的方法,对采自石河子地区的29个黄萎病菌系进行培养特性观察、致病力测定和特异性引物PCR检测,分析不同致病类型菌系的致病力及接种后落叶情况。【结果】各供试菌系的生长速度存在一定差别,其菌落形态主要为菌核型和菌丝型,分别占75.9%和17.2%。鉴别寄主法测定表明,供试菌系存在明显的致病性分化,强、中、弱菌系分别占51.7%、31.0%和17.3%。致病型测定显示,供试菌系中落叶型菌系和非落叶型菌系分别占37.9%和58.6%,1个菌系未检测出致病类型。落叶型菌系的致病性明显强于非落叶型菌系,其平均病情指数分别为39.5和27.6,二者接种后的落叶程度虽有明显差异,但都可以产生落叶症状,其落叶症状的轻重不仅与菌系的致病类型有关,同时与品种的抗病性也密切相关。【结论】石河子地区棉花黄萎病菌的菌落形态主要以菌核型为主。落叶型菌系的致病力明显高于非落叶型菌系,强致病力落叶型菌系的明显增多是当前病害明显加重的原因之一。
译  名：
Study on the Culture Characteristic and Pathogenic Differentiation of Veticillium dahliae from Cotton in Shihhotze
作  者：
HAN Hong-wei,REN Yu-zhong,GAO Feng,LIU Pei-yuan,JI Li-li,LI Chun,LI Guo-ying(The Key Laboratory of Prevention and Control for Oasis Crop Disease,Shihhotze University,Shihhotze Xinjiang 832000,China)
关键词：
cotton;Verticillium dahliae;culture characteristic;pathogenicity differentiation;symptoms of defoliation;Shihhotze
摘  要：
【Objective】This research focused on the pathogenic differentiation of Veticillium dahliae from Cotton in Shihezi to reveal the reason of disease explosion on pathogenic aspect.【Method】29 representative strains were collected and observed by culture characteristic and determined by pathogenicity type base on differential host test,PCR assay and the defoliate symptoms.【Result】The colony of strains differed in growth rates,and these sclerotium and filament type were domain,75.9% and 17.2% separately.The results of inoculating differential host test showed that strong,medium and weak pathogenicity strains occupied 51.7%,31.0% and 17.3% of total examined strains,respectively.However the defoliating and non-defoliating strains occupied 37.9% and 58.6% of them,and the corresponding average index was 39.5 and 27.6,respectively.Defoliating symptoms was observed in plants inoculated both defoliating and non-defoliating strains,but severity have manifest difference.Thus,the defoliating symptom is not only related with the pathogenicity and pathogenicity type of pathogens,but also with the resistance of cotton cultivars.【Conclusion】The sclerotium type strains are the majority in Shihezi.The pathogenicity of defoliating strains is obvious stronger than that of non-defoliating strains,and the explosion of the strong pathogenicity and defoliating population is one of the key factor of disease increasing visibly in filed.

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