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Position: Home > Articles > Cloning and Sequencing of Raccoon dog IL-18 gene Special Wild Economic Animal and Plant Research 2008,30 (3) 20-24

貉白介素-18(IL-18)基因的克隆及序列分析

作  者:
张海玲;罗国良;赵春霏;柴秀丽;王凤雪;易立;闫喜军
单  位:
中国农业科学院特产研究所
关键词:
貉;IL-18;克隆;序列分析
摘  要:
从植物血凝素(PHA)诱导的貉外周血淋巴细胞中提取总RNA,通过反转录聚合酶链反应(RT-PCR)首次对貉IL-18的基因进行扩增。序列分析结果表明,该序列含有1个582bp的开放阅读框架,编码193个氨基酸,与已报道的北极狐和犬IL-18序列同源性高达95.9%和94.8%,氨基酸同源性为93.8%和91.2%,与其它物种的IL-18基因间具有较大种属差异性。
译  名:
Cloning and Sequencing of Raccoon dog IL-18 gene
作  者:
ZHANG Hai-ling,LUO Guo-liang,ZHAO Chun-fei,CHAI Xiu-li, WANG Feng-xue,Yi Li,YAN Xi-jun(Institute of Special Wild Animal and Plant Science,CAAS.,Jilin 132109,China)
关键词:
Raccoon dog;Interleukin-18;Cloning;Sequencing
摘  要:
To provide a basis for further investigation of the function of Raccoon dog IL-18,a pair of primers specific to Raccoon dog Interleukin-18(IL-18) were designed and synthesized according to the known dog IL-18 sequence from GenBank.Raccoon dog IL-18 cDNA were used to amplify by RT-PCR from total RNA of Raccoon dog PBMC cells induced with PHA.RT-PCR product was cloned into pMD18-T vector.The nucleotide acid sequence was determined after being identified by restriction endonuclease digestion.The result showed that cloned Raccoon dog IL-18 cDNA was 590 bp in length with a 582 bp open reading frame encode a polypeptide of 193 amino acid.Compared with published arctic fox and dog IL-18,nucleotide acid homology were 95.9 % and 94.8%,and amino acid homology were 93.8% and 91.2%,respectively.There is significant difference between Raccoon dog and other species of IL-18 gene sequence.The obtaining of IL-18 sequence laid the foundation for its further study and exploitation.

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