Position: Home > Articles > Influence of different hormone combinations on Kentucky bluegrass ‘ABBY’ Callus induction
Pratacultural Science
2013,30
(12)
67-72
不同激素配比对“ABBY”草地早熟禾愈伤组织诱导的影响
作 者：
蒋新民;杨春华;刘羽
单 位：
四川农业大学动物科技学院草业科学系
关键词：
ABBY草地早熟禾;成熟种子;组织培养;植株再生
摘 要：
以结实率高的草地早熟禾(Poa pratensis)品种"ABBY"成熟种子为外植体,利用MS基本培养基为介质,探索不同浓度2,4-D和6-BA对其愈伤组织诱导及分化的影响。结果表明,MS+1.0 mg·L-1 2,4-D+0.2mg·L-16-BA的胚性愈伤组织出愈率最高,达54.74%;MS+1.0mg·L-12,4-D+0.4mg·L-1 6-BA的胚性愈伤组织出愈率稍低,为45.81%,但愈伤组织质量最好。愈伤组织分化2~3代后,MS+3mg·L-16-BA+0.2mg·L-1 NAA的愈伤组织分化率最高,达100%,且分化率明显受愈伤组织培养时间及培养环境的影响。
译 名：
Influence of different hormone combinations on Kentucky bluegrass ‘ABBY’ Callus induction
作 者：
JIANG Xin-min;YANG Chun-hua;LIU Yu;Grassland Science,College of Animal Science & Technology,Sichuan Agriculture University;
关键词：
Kentucky bluegrass‘ABBY’;;mature seed;;tissue culture;;plant regeneration
摘 要：
The present study describes callus induction of the plant‘ABBY'Kentucky bluegrass(Poa pratensis)using mature seeds as explants and the influence of different combinations of hormone:2,4-D ichlorophenoxyacetic acid(2,4-D)and 6-Benzylamin(6-BA)with Murashige & Skoog Basal Medium(MS)as the basic medium on callus initiation and subsequent growth.The results showed that the hormone combination:MS+1.0 mg·L-1 2,4-D+0.2 mg·L-1 6-BA has highest callus induction rate(54.74%).The hormone combination MS+1.0mg·L-12,4-D+0.4mg·L-16-BA has the best quality callus although the callus induction rate is lower(45.81%).The hormone combination:MS+3.0 mg·L-16-BA+0.2mg·L-1 NAA was suggested to be the best differentiation medium with a callus differentiation rate at 100%after 2~3generations.The differentiation rate was affected by callus condition and incubation time.
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