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当前位置: 首页 > 文章 > 液相色谱-串联质谱法测定牛奶中粘杆菌素和多粘菌素B的残留量 现代农业科技 2013 (22) 260-261,268
Position: Home > Articles > 液相色谱-串联质谱法测定牛奶中粘杆菌素和多粘菌素B的残留量 Modern Agricultural Science and Technology 2013 (22) 260-261,268

液相色谱-串联质谱法测定牛奶中粘杆菌素和多粘菌素B的残留量

作  者：
梁雪琪;刘红;何凤芹
关键词：
液相色谱-串联质谱法;残留量;粘杆菌素;多粘菌素B
摘  要：
建立了同时测定牛奶中粘杆菌素和多粘菌素B素残留量的液相色谱-串联质谱(LC-MS/MS)的方法.样品通过5%高氯酸溶液沉淀蛋白,经乙醚除脂,Strata-X固相萃取柱净化,浓缩后经LC-MS/MS电喷雾正离子多反应检测模式(ESI+MRM)检测.结果显示,粘杆菌素和多粘菌素B在25~1 000 ng/mL范围内均具有良好的线性关系,相关系数不低于0.997 2,加标回收率为71.5%~98.1%,相对标准偏差为3.8%~9.5%.试验证明该方法前处理简单,快速,稳定性好,适用于大量样品中粘菌素和多粘菌素B残留的同时检测.
单  位：
Zhongshan Agricultural Products Quality Supervision & Inspection Institute in Guangdong Province
关键词：
LC-MS/MS%residues%colistinin%polymycin B
摘  要：
A method was established to simultaneously detect colistinin and polymycin B in milk by liquid chromatography tandem mass spetrometry(LC-MS/MS).The samples,protein was precipitated with 5% perchloric acid and the fat was removed with diethyl ether,the other impurities were removed with Strata-X solid phase extraction cartridge,after concentration,theanalvtes were detected by LC-MS/MS(ESI)with multiple reaction monitoring(MRM).The results indicated that the calibration curves were in good linearity between the peak area and the concentration of 25~1 000 ng/mL for colistinin and polymycin B with correlation cofficients not less than 0.997 2,the spiked recoveries ranged from 71.5%~98.1% with reative standard deviations of 3.8% ~9.5%.The proposed method was simple and rapid,the stability was good and it was suitable for the simultaneous quantitative detection of colistinin and polymycin B in milk.

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