Position: Home > Articles > Determination and Purification of Polysaccharides in Fruit of Quince
FOOD SCIENCE
2008,29
(3)
229-232
榅桲果实中多糖含量测定及纯化
作 者：
敬思群;关延新
单 位：
新疆大学生命科学与技术学院;新疆巴州质量技术监督局检验所
关键词：
榅桲多糖;苯酚-硫酸法;含量测定;纯化
摘 要：
用水提醇沉法提取榅桲多糖,应用分光光度法对榅桲果实中的多糖含量进行了测定。经苯酚-硫酸显色,490nm处测定,其多糖的含量为67.28%,RSD为3.08%(n=4)。该测定方法重现性较好,平均回收率为101.2%±3.54%,RSD为3.50%(n=3)。多糖浓缩液经聚酰胺柱纯化,脱色效果明显。
译 名：
Determination and Purification of Polysaccharides in Fruit of Quince
作 者：
JING Si-qun1,GUAN Yan-xin2 (1.College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China; 2.Institute of Testing, Xingiang Bazhou Qulity Technology Supervision Bureau, Bazhou 841000, China)
关键词：
quince polysaccharide;phenol-vitriolic;determination;purification
摘 要：
Polysaccharides from fruit of quince was obtained by hotwater extraction and ethanol precipitation. The content of polysaccharide was determined by spectrophotometry.The content of polysaccharide assayed by phenol-vitriolic colorimetry at 490nm,was 67.28% with3.08% of RSD (n=4). Recovery value for the polysaccharides assayed was 101.2%. The concentrated polysaccharides was purified by polyamide column and the results showed that decolorization was significant.
导出引用
计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
