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当前位置: 首页 > 文章 > 黄壤溶磷青霉菌的筛选及培养条件优化 河南农业科学 2019,48 (5) 56-61
Position: Home > Articles > Screening and Optimization of Culture Conditions for Phosphate Solubilizing Penicillium in Yellow Soil Journal of Henan Agricultural Sciences 2019,48 (5) 56-61

黄壤溶磷青霉菌的筛选及培养条件优化

作  者：
乔志伟
单  位：
安顺学院 资源环境与工程学院
关键词：
黄壤;溶磷青霉菌;溶磷特性;正交试验
摘  要：
为深入挖掘溶磷真菌在提高黄壤磷素有效性方面的作用,通过溶磷真菌筛选培养基(PVK)平板分离法从贵州安顺黄壤农田作物根际土壤中分离筛选出8株溶磷真菌.经过多次的分离纯化后,得到1株溶磷能力较强的真菌菌株G8,结合该菌株菌落形态特征和18 S rRNA基因序列分析,确定其为青霉菌(Penicillium sp.),进一步研究了G8的溶磷特性及最佳培养条件.结果表明:G8以葡萄糖为碳源、硫酸铵为氮源的条件下,在磷酸三钙培养液中有效磷含量最高,为534.24 mg/L.G8在磷酸铝培养液中有效磷含量在第6天达到最大,为589.74 mg/L;G8在磷酸铁培养液中有效磷含量在第5天达到最大,为201.38 mg/L.G8在磷酸铁和磷酸铝培养液中,有效磷含量与pH值呈显著的负相关(P<0.05).通过正交试验得到G8最佳培养条件为葡萄糖含量15 g/L、接种量1%(体积比)、培养液最初pH值6、硫酸铵含量0.067 g/L.培养条件优化后G8的溶磷能力明显增强.
译  名：
Screening and Optimization of Culture Conditions for Phosphate Solubilizing Penicillium in Yellow Soil
作  者：
QIAO Zhiwei;College of Resources and Environment Engineering,Anshun College;
关键词：
Yellow soil;;Phosphate-solubilizing penicillium;;Phosphate solubilizing characteristics;;Orthogonal test
摘  要：
To further explore the function of phosphate solubilizing fungi in improving phosphorus availability in yellow soil, eight phosphate solubilizing fungi strains were isolated and screened from the crop rhizosphere of yellow soil in Guizhou province by phospholytic fungi screening medium(PVK) plate method.After repeated isolation and purification,the strain G8 with strong phosphate-solubilizing activity was obtained,and identified as Penicillium sp.,based on the morphological characteristics and the analysis of 18 S rRNA gene sequence,then further studied phosphate solubilizing characteristics of G8 and the optimal culture conditions.The results showed that,under the condition of glucose as carbon source,and ammonium sulfate as nitrogen source,G8 had the highest available phosphorus content in tricalcium phosphate culture solution,which was 534.24 mg/L.The available phosphorus content of G8 in aluminium phosphate culture solution reached the maximum on the 6 th day,which was 589.74 mg/L.In ferric phosphate culture solution,the available phosphorus content of G8 reached the maximum on the 5 th day,which was 201.38 mg/L.There was a significant negative correlation between available phosphorus content and pH value in iron phosphate and aluminium phosphate culture solution(P<0.05).Orthogonal experiments showed that,the optimum conditions of G8 were glucose content 15 g/L,inoculation amount 1%(volume ratio),initial pH value 6 and ammonium sulfate content 0.067 g/L.The phosphorus solubility of G8 was enhanced after the culture conditions were optimized.

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