中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 云南地方稻种抗稻瘟病基因Pi-d3序列变异分析中国水稻科学 2016,30 (1) 17-26

Position: Home > Articles > Sequence Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace Chinese Journal of Rice Science 2016,30 (1) 17-26

云南地方稻种抗稻瘟病基因Pi-d3序列变异分析

作者：

杨奕;孙一丁;马继琼;王炎炎;许明辉

单位：

云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室

关键词：

云南地方稻种;稻瘟病;Pi-d3基因;单核苷酸多态性

摘要：

对来自云南的80份地方稻种抗稻瘟病基因Pi-d3编码区序列进行了PCR扩增并测序分析。结果表明,与地谷相比,80份云南地方稻种的Pi-d3编码区序列(全长2775bp)存在39个变异位点,平均变异率为1.41%。所有材料可以归为37种单倍型,频率较高的单倍型是H8(28.8%)、H4(11.3%)、H23(5.0%),其他单倍型频率较低,显示了云南地方稻种Pid3基因变异丰富,单倍型类型多,但频率较低。37种单倍型中存在28个氨基酸差异位点,共编码33种蛋白,其中18种单倍型共计32份材料(包括12份籼稻品种)出现了假基因,假基因化的频率较高,可能是粳稻Pi-d3假基因化向籼稻渗透。除已发现的737位氨基酸假基因位点外,新发现32和467两个假基因位点。Pi-d3基因在籼稻、粳稻亚种,水稻、陆稻,黏稻、糯稻的单倍型类型及频率两方面均存在差异,表明亚种间或生态型间发生了一定的遗传分化。37种单倍型在云南的地理分布呈大分散、小聚居的特点,普洱、版纳、临沧的单倍型种类最丰富,并以之为中心向外扩展,单倍型种类随之减少。

译名：

Sequence Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace

作者：

YANG Yi;SUN Yi-ding;MA Ji-qiong;WANG Yan-yan;XU Ming-hui;Institute of Biotechnology and Genetic Resources,Key Laboratory of Biotechnology Research of Yunnan Province,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture,Yunnan Academy of Agricultural Sciences;

关键词：

Yunnan rice landraces;;rice blast;;Pi-d3;;single nucleotide polymorphism

摘要：

The sequence variations of Pi-d3 for rice blast resistance in 80 Yunnan rice landraces were analyzed by resequencing open reading frame(ORF).Compared with Pi-d3 Digu ORF,a total of 39 nucleotide variations were found in2775-bp ORFs of 80 rice accessions with the average mutation rate of 1.41%.The WTBXPi-d3 ORF of 80 rice accessions was divided into 37 haplotypes based on the nucleotide variations.H8(28.8%),H4(11.3%)and H23(5.0%)are high-frequency types,while other types occurred at low frequency.The results showed that the WTBXPi-d3 in Yunnan rice landraces was rich in genetic variations and haplotypes,but low frequency.A total of 28 amino acids variations were found in 33 translation proteins of 37 haplotypes.18haplotypes with 32 rice landraces including 12 indica accessions possessde the Pi-d3 pseudogenes.The frequency of pseudogene was high.It was possible due to that the Pi-d3 pseudogene permeated to indica.Terminators were newly founded at position 32 and 467of protein except the published terminator at position 737.Pi-d3 differed in both type and frequency of haplotypes between the subspecies of indica and japonica,the ecotypes of lowland and upland rice,no-waxy and waxy rice.It indicated that genetic differentiation occurred between subspecies or ecotypes.Geographical distribution showed that 37 haplotypes spread over a large area while concentrated in small areas.Puer,Banna and Lincang were the central area with abundant haplotypes,and based on this scale,the types of haplotypes decreased outward progressively.

相似文章

两个时期收集的云南水稻农家品种表型多样性比较 [李金梅, 崔迪, 汤翠凤, 阿新祥, 余滕琼, 马小定, 张恩来, 刘昌文, 徐福荣] 植物遗传资源学报 2015,16 (2) 238-244
云南地方稻种的多样性及优异种质研究 [曾亚文, 李自超, 申时全, 王象坤, 杨忠义, 张洪亮, 陈于敏] 中国水稻科学 2001,15 (3)
云南地方稻核心种质孕穗期耐冷性及其地理分布 [李绅崇, 曾亚文, 申时全, 李本逊, 张忠省, 戚六花, 普晓英] 中国水稻科学 2004,18 (5)
籼粳RILs孕穗期耐冷性研究 [凌青, 曾亚文, 申时全, 普晓英, 程德兵, 罗龙] 西南农业学报 2004,17 (1) 40-43
云南地方稻核心种质利用研究Ⅰ.农艺性状的表现及遗传多样性 [申时全, 普晓英, 杜娟, 曾亚文] 西南农业学报 2006,19 (1) 22-29
云南稻种核心种质耐低磷性状间的相关性分析 [杜娟, 曾亚文, 普晓英, 申时全, 杨树明, 刘昆, 杨晓娟] 西南农业学报 2006,19 (1) 30-33
云南地方稻种灿粳亚种的生态群分类及其地理生态分布 [曾亚文, 李自超] 作物学报 2001,027 (1) 15-20
云南地方稻种籼粳亚种的生态群分类及其地理生态分布 [曾亚文, 李自超, 杨忠义, 申时全, 张洪亮, 王象坤] 作物学报 2001,27 (1) 15-20
云南稻核心种质F_2世代形态多样性鉴定与分类 [杨树明, 曾亚文, 杜娟, 杨涛, 普晓英, 杜威, 夏小环, 普正贵, 方晓东] 湖南农业大学学报(自然科学版) 2008,34 (5) 5-9
利用与抗稻瘟病基因Pil连锁的MRG4766标记鉴定173份云南地方稻种 [李进斌, 李鼎, 孙一丁, 许明辉] 分子植物育种 2012,10 (1) 73-79

导出引用

计量

文章访问数: 6

HTML全文浏览量: 0

PDF下载量: 0

云南地方稻种抗稻瘟病基因Pi-d3序列变异分析

相似文章

所属期刊

中国水稻科学

推荐期刊

中国水稻科学

西南农业学报