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Position: Home > Articles > The Development of the Rice Blast Resistance Genes Pi2-InDel Markers and Its Evaluation in Application Molecular Plant Breeding 2017,15 (2) 594-598

水稻稻瘟病抗性基因Pi2-InDel标记的开发与评价

作  者:
杨立明;纪剑辉;周颖君;方继朝;刘永锋
单  位:
淮阴师范学院生命科学学院江苏省环洪泽湖生态农业生物技术重点实验室江苏省区域现代农业与环境保护协同创新中心江苏省农业科学院植物保护研究所;淮阴师范学院生命科学学院;淮安市清浦区农技推广中心
关键词:
水稻;稻瘟病抗性基因;Pi2-插入/缺失标记;Pi2/9位点
摘  要:
通过对水稻抗稻瘟病基因Pi2/9位点上已克隆的功能基因及非功能位点序列的相互对比,鉴定到Pi2特异的核苷酸差异,开发了基于PCR技术的Pi2基因的插入/缺失(insert-deletion,InDel)标记,能有效地将Pi2与其它抗性等位基因及感病等位基因区分开。利用Pi2基因的分离群体进行标记选择与接种鉴定,结果表明Pi2-InDel标记能精确地选择Pi2基因。用Pi2-InDel标记对20份水稻品种和育种亲本进行分子检测,该标记能准确地将抗性基因分离开来。这为筛选水稻稻瘟病抗性种质资源,以及抗性标记辅助抗病育种提供了便利。
译  名:
The Development of the Rice Blast Resistance Genes Pi2-InDel Markers and Its Evaluation in Application
作  者:
Yang Liming;Ji Jianhui;Zhou Yingjun;Fang Jichao;Liu Yongfeng;Luo Yuming;School of Life Science,Huaiyin Normal University,Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake,Jiangsu Col laborative Innovation Center of Regional Modern Agriculture & Environmental Protection;Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences;
关键词:
Rice;;Blast resistance gene;;Pi2-InDel;;Pi2/9 locus
摘  要:
The molecular markers, designated as Pi2-InDel, for detecting rice alleles of Pi2 has been successfully developed through sequence comparison strategy. This specific marker Pi2-InDel can obviously distinguish Pi2 gene from others mapped on Pi2/9 locus. With Pi2-InDel marker, 20 representative cultivars and breeding materials can be diagnosed. Moreover, this marker can accurately selected out the Pi2 gene, which facilitates the screening and identification of resistant germplasm to rice blast, and molecular marker assisted breeding for rice blast resistance.

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