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当前位置: 首页 > 文章 > 利用表面等离子共振技术快速检测牛奶中的磺胺甲噁唑 食品科学 2010,31 (6) 168-171
Position: Home > Articles > Surface Plasmon Resonance for Rapid Determination of Sulfamethoxazole in Milk FOOD SCIENCE 2010,31 (6) 168-171

利用表面等离子共振技术快速检测牛奶中的磺胺甲噁唑

作  者：
周宏敏;欧惠超;姜浩;江海峰;王晓萍;罗昭锋
单  位：
合肥微尺度物质科学国家实验室;浙江大学光电信息工程学系
关键词：
表面等离子共振;芯片;磺胺甲噁唑
摘  要：
利用表面等离子共振(SPR)技术,建立快速定量检测牛奶中磺胺甲噁唑(SMX)的方法。将SMX共价偶联到表面等离子共振芯片表面,并对抗体的结合浓度及芯片的再生条件进行优化,检测芯片的稳定性。在无抗牛奶中添加系列质量浓度的SMX,利用免疫竞争抑制原理构建标准曲线,并对市售18个牛奶样品进行检测。结果表明:制备的芯片稳定,90个循环相对标准偏差(RSD)为1.23%;该方法的检测限为3.2ng/mL;18个牛奶产品中16个SMX的残留量在规定的允许范围内。所建立的方法可以在15min内完成样品的前处理和检测,是一种简便快捷的定量检测方法。
译  名：
Surface Plasmon Resonance for Rapid Determination of Sulfamethoxazole in Milk
作  者：
ZHOU Hong-min1,OU Hui-chao1,JIANG Hao1,JIANG Hai-feng1,WANG Xiao-ping2,LUO Zhao-feng1,*(1.Hefei National Laboratory for Physical Sciences at Microscale,School of Life Sciences,University of Science and Technology of China,Hefei 230027,China;2.Department of Optical Engineering,Zhejiang University,Hangzhou 310027,China)
关键词：
surface plasmon resonance;chip;sulfamethoxazole
摘  要：
A method based on surface plasmon resonance technique was developed for rapid determination of sulfamethoxazole(SMX) in milk.SMX was covalently coupled to laboratory prepared CM5 chip to obtain SMX chip.The optimal antibody working concentration and chip regeneration conditions were investigated.Meanwhile,the stability of SMX chip was measured.A series of different concentrations of SMX was added to blank milk samples to construct a standard curve for SMX quantification according to the principle of competitive immune inhibition assay.Eighteen commercial milk samples were determined by the developed method.SMX chip displayed good stability,and the relative standard deviation(RSD) for 90 cycles was 1.23%.The limit of detection(LOD) of SMX was 3.2 ng/mL.SMX contents below the required threshold level were observed in 16 of 18 milk samples.Less than 15 min was consumed for the determination of SMX by the developed method.Thus,this method may offer a simple and efficient approach for SMX quantification.

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