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Position: Home > Articles > Detection of DNA polymorphism among Fasciola hepatica,Fischoederius elongates and Schistosoma japonicum Chinese Journal of Veterinary Medicine 2001,37 (12) 8-9

肝片形吸虫长菲策吸虫及日本血吸虫DNA的多态性检测

作  者:
胥全彬;沈永林;刘耀兴
单  位:
南京农业大学动物医学院;江苏省畜牧局
关键词:
肝片形吸虫;长菲策吸虫;日本血吸虫;RAPD特征条带
摘  要:
本试验用随机扩增 DNA多态性技术对肝片形吸虫、长菲策吸虫和日本血吸虫虫体总 DNA的多态性进行检测 ,结果筛选出三条具有种鉴别意义的 RAPD谱带。这些特征性条带可望标记成探针用于粪便学检查中虫卵的鉴定
译  名:
Detection of DNA polymorphism among Fasciola hepatica,Fischoederius elongates and Schistosoma japonicum
作  者:
XU Quan bin 1,SHEN Yong lin 1,LIU Yao xing 2 (1. College of Vaterinary Medicine Nanjing Agricultural University, Nangjing 210095, China; 2. Jiangsu Animal Husbandry Bureau, Nanjing 210024, China)
关键词:
Fasciola hepatica; Fischoederius elongatus; Schistosoma japonicum; species specific RAPD patterns
摘  要:
The polymorphic DNA of Fasciola hepatica Fischoederius elongates , and Schistosoma japonicum were detected with RAPD in this assay. After amplification with six primers, three species specific RAPD patterns were found, which could be used as a probe for identification of eggs in feces examination.

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