当前位置: 首页 > 文章 > SPA-ELISA监测猪传染性胃肠炎病毒血清抗体水平的研究 中国动物检疫 2003,20 (8)
Position: Home > Articles > Studies on Application of SPA-ELISA for Detectin of Serum Antibody to Transmissible Gastroenteritis China Animal Health Inspection 2003,20 (8)

SPA-ELISA监测猪传染性胃肠炎病毒血清抗体水平的研究

作  者:
姜迪来;郭福生;尹燕博;徐兰芳
单  位:
农业部动检所;江西农业大学;山东淄博出入境检验检疫局
关键词:
TGEV;PRCV;SPA-ELISA;IFA
摘  要:
建立了SPA -ELISA监测猪传染性胃肠炎病毒 (TGEV)血清抗体水平的方法 ,与免疫荧光试验 (IFA)符合率高达90.9%。试验表明 :病毒抗原最适包被浓度为4μg/ml;血清工作浓度为1∶200;最佳包被液采用0.05M pH9.6的碳酸缓冲液 ;封闭液选用2 %的脱脂奶 ;并确定了抗原抗体最佳反应时间和最适反应温度及底物溶液的显色时间。特异性试验表明所组装的SAP -ELISA试剂盒特异性较好 ,可用于TGE的流行病学调查。
译  名:
Studies on Application of SPA-ELISA for Detectin of Serum Antibody to Transmissible Gastroenteritis
作  者:
Jiang Di-lai 1) ,Guo Fu-sheng 2) ,Yin Yan-bo 2) ,Xu Lan-fang 3) (1.Zibo Exit&Entry Inspection and Quarantine Service,255031; 2.National Animal Quarantine Institute,MOA,Qingdao,266032; 3.Jiangxi Agricnltunal university,Nanchang330045)
关键词:
SPA-ELISA,TGEV,Antibody Detection
摘  要:
An SPA-ELISA was developed for detection of serum antiboy against transmissible gastroenteritis virus,its coincidence with IFA was90.9%.Tests indicated that the optimal antigen coating concentration was4μg/ml;working serum concentration was1∶200;the best coating solution was0.05M carbonate buffer(pH9.6)and2%skimmed milk was used as the blocking solution.The antigen-antibody reaction was best at37℃for60min and20min was reˉquired for colur development.The assempled SPA-ELISA kits are suitable for field TGE epidemiological investigation.

相似文章

计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊