作 者：
王璟;杨敬林;廖健程;丁鑫;陈哲;张立成
单 位：
福建省农业科学院土壤肥料研究所;湖南农业大学工学院
关键词：
稻-稻-油菜轮作;枯草芽孢杆菌;荧光定量PCR;土壤环境
摘 要：
为检测长期进行稻-稻-油菜轮作土壤中枯草芽孢杆菌的数量变化,以长期稻-稻连作土壤为对照比较。在连续30年轮作和连作的大田(湖南安仁试验基地)进行试验,应用荧光定量PCR技术,对两类土壤中的枯草芽孢杆菌的数量进行检测。试验于2015年7月、10月和2016年4月3个时期对定点试验土壤进行取样。通过枯草芽孢杆菌特异性引物进行荧光定量PCR扩增。3个时期采样土壤的检测结果:轮作土壤中枯草芽孢杆菌数量比连作土壤分别增加37.50%,27.06%和20.20%;轮作和连作2015年7月采样土壤中枯草芽孢杆菌数量大于2016年4月采样土壤测定值,大于2015年10月采样土壤测定值。研究结果表明稻-稻-油菜轮作提高了土壤枯草芽孢杆菌的数量,使土壤中对作物生长有益的特定功能微生物数量增加,有利于改善土壤生态环境。
译 名：
Fluorescent quantitative PCR analysis of Bacillus subtilis in rice-rice-rape rotation system
作 者：
WANG Jing;YANG Jing-lin;LIAO Jian-cheng;DING Xin;CHEN Zhe;ZHANG Li-cheng;Hunan Agricultural University Engineering College;Soiland Fertilizer Institute,Fujian Academy of Agricultural Science;
单 位：
WANG Jing%YANG Jing-lin%LIAO Jian-cheng%DING Xin%CHEN Zhe%ZHANG Li-cheng%Hunan Agricultural University Engineering College%Soiland Fertilizer Institute,Fujian Academy of Agricultural Science
关键词：
rice-rice-rape rotation cropping;;Bacillus subtilis;;fluorescent quantitative PCR;;soil environment
摘 要：
In order to test the number of Bacillus subtilis in soil under the long?term rice?rice?rape rotation condition,taking long?term rice?rice rotation cultivation as the control,acomparison between the soils of the two cultivating types in Anren Hunan was made the soils were measured by quantitative PCR,one was the rice?rice?rapeseed rotation soil and the other was rice?rice continuous cropping soil,which were both cultivated for 30 years.The soil samples were taken in the fixed?point test spot in July,2015 and October,2015 and April,2016.The special primers of Bacillus subtilis were amplified with fluorescent quantitative PCR.The results of testing the soils sampled in the 3 periods showed that the Bacillus subtilis of the rice?rice?rapeseed rotation soil,cmpared with the rice?rice continuous cropping soil,increased by 37.50% and 27.06% and 20.20% respectively.The number of Bacillus subtilis of the rice?rice?rapessd rotation soil and the rice?rice continuossoilsampled in July,2015 wasgreaterthanthatofthesoilsampledinOctober,2015,andbothweregreaterthanthatofthesoilsampled in April,2016.The cultivation of rice?rice?rape rotation can improve the number of Bacillus subtilis and then im?provethemicroecologicalenvironmentof soil.
