中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 家蝇胚胎细胞系染色体制作方法畜牧与饲料科学 2011 (7) 1-2

Position: Home > Articles > Preparation Methods of the Chromosome of Cell Lines in Musca domestica Embryo Animal Husbandry and Feed Science 2011 (7) 1-2

家蝇胚胎细胞系染色体制作方法

作者：

Xi Zhan

单位：

Staff Room of Parasitology,Zunyi Medical College,Zunyi 563003,China

关键词：

chromosome;colchicine;mdec;07114;pre;fixativ

摘要：

[目的]探索家蝇胚胎细胞系(MDEC-07114)染色体制作的方法。[方法]以MDEC-07114细胞为材料制备染色体。实验分组:秋水仙素浓度为5、10mg/L组;秋水仙素处理时间为5、6、10h组;预固定组和直接固定组。[结果]秋水仙素浓度为10mg/L、作用时间为5h组,细胞分裂相多,染色体形态好;预固定处理后细胞分散好,染色体形态清晰。[结论]10mg/L秋水仙素作用5h,经预固定处理后制作MDEC-07114细胞染色体效果最佳。

译名：

Preparation Methods of the Chromosome of Cell Lines in Musca domestica Embryo

作者：

HE Li-fang,ZHAO Dan,LIU Hui,HUANG Xue-gui,ZHANG Xi (Staff Room of Parasitology,Zunyi Medical College,Zunyi 563003,China)

关键词：

embryo;cell line;chromosome;Musca domestica

摘要：

[Objective] The research aimed to explore the preparation method of chromosome of cell lines(MDEC-07114) in Musca domestica embryo. [Method] The chromosome was prepared with MDEC-07114 cells as materials. The experimental groups were set up as follows:groups of 5 and 10 mg/L colchicine;groups of treating with colchicine for 5,6 and 10 h,pre-fixative group and the fixed group. [Result] More cell division and good chromosome morphology can be observed in the group of treating with 10 mg/L colchicine for 5 h. And clear and separated chromosome were observed in pre-fixative group. [Conclusion] The prepared chromosome of MDEC-07114 cells after treating with 10 mg/L colchicine for 5 h and pre-fixative treatment had the best effects.

相似文章

Construction of Molecular Linkage Map of Cultivated Allotetraploid Cotton (Gossypium hirsutum L.*G. barbadense L. )with SSR and RAPD Markers [] 棉花学报 2002 16
Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat [Meng Ya-ning, Kang Su-hua, Lan Su-que, Li Xing-pu, Zhang Ye-lun, Bai Feng] 农业科学学报 (英文) 2013,12 (5)
小鼠骨髓染色体G显带最佳制备方法的探究 [Ye X] 畜牧与饲料科学 2012,33 (1) 27-28
Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat [Yan Meng, Su-hua Kang, Shuiyun Lan, Xingpu Li, Yelun Zhang, Bin Fen] 农业科学学报(英文版) 2013,12 (5)
Karyotype analysis on chromosome of Curcuma kwangsiensis S. G. Lee et C. F. Liang. [Jiling Huang, Zhao YingXue, Xihua Liu, Jian Wan] 药用植物研究(英文版) 2010,1 (9)
Genetics of Populations of Anopheles maculipennis. Preliminary Studies on Chromosome Patterns in the Group. [G. Frizz] 水稻科学(英文版) 1949,19 (6)
Karyotype Analysis of Gazania rigens Varieties [Jiashi Zeng, Dongxu Wang, Yangqing Wu, Xuesong Guo, Yazhou Zhang, Xiaoping Lu] 园艺学报 2016,2 (5) 279-283
Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut [Kunkun Zhao, Xiongming Du, Jingjing Zhang, Yi Fan, Qian Ma, Yaoyao Li, Zenghui Cao, Di Cao, Sasa Hu, Ding Qiu, Kai Zhao, Fangping Gong, Zhong‐Feng Li, Rui Ren, Lin Zhang, Xingli Ma, Xing‐Guo Zhang, Dongmei Yi] 作物学报(英文版) 2024

导出引用

计量

文章访问数: 14

HTML全文浏览量: 0

PDF下载量: 0

家蝇胚胎细胞系染色体制作方法

相似文章

所属期刊

畜牧与饲料科学

推荐期刊

棉花学报

药用植物研究(英文版)