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Position: Home > Articles > Effects of Turmeric rhizome Naphtha on Gene Expression of Hepatocarcinoma Cell SMMC-7721 FOOD SCIENCE 2009,30 (19) 240-243

莪术油对肝癌细胞SMMC-7721基因表达的影响

作  者：
王顺启;陈力;倪虹;穆亚丽;刘玮;谢明勇
单  位：
南昌大学计算机技术工程研究所;南昌大学生命科学与食品工程学院;南昌大学;南开大学生命科学学院
关键词：
莪术油;肝癌细胞SMMC-7721;MTT比色法;微阵列;基因表达
摘  要：
目的:检测莪术油对肝癌细胞SMMC-7721生长,及肝癌相关基因表达的影响。方法:利用MTT比色法检测莪术油对肝癌细胞SMMC-7721增殖的影响;利用以肝癌cDNA抑制性消减文库构建的肝癌相关基因微阵列,检测莪术油作用肝癌细胞SMMC-772136h后基因的表达变化。结果:莪术油作用肝癌细胞SMMC-772136h后,细胞生长受到明显抑制,并观察到有23个基因表达下调,3个基因表达上调。结论:莪术油对肝癌肿瘤细胞的作用是通过影响多基因的表达改变实现的。
译  名：
Effects of Turmeric rhizome Naphtha on Gene Expression of Hepatocarcinoma Cell SMMC-7721
作  者：
WANG Shun-qi1,2,3,CHEN Li2,NI Hong2,MU Ya-li4,LIU Wei3,XIE Ming-yong1,(1. State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2. College of Life Science,Nankai University,Tianjin 300071,China;3. School of Life Sciences and Food Engineering,Nanchang University,Nanchang 330031,China;4. Institute of Computer Technology and Engineering,Nanchang University,Nanchang 330031,China)
关键词：
Turmeric rhizome naphtha (TRN);hepatocarcinoma cell SMMC-7721;MTT assays;microarray;gene expression
摘  要：
Objective:To detect effects of Turmeric rhizome naphtha (TRN) on cell growth and gene expression of hepatocarcinoma cell SMMC-7721. Methods:Cell growth of hepatocarcinoma cell SMMC-7721 with TRN treatment was evaluated by MTT assays,and gene expression of hepatocarcinoma cell SMMC-7721 with TRN treatment for 36 hours was explored with microarray of hepatocarcinoma-associated genes,which resulted from suppression subtractive hybridization library. Results:After TRN treatment on hepatocarcinoma cell SMMC-7721 for 36 hours,cell growth was greatly inhibited and gene expression profiling analysis indicated that 23 genes were up-regulated and 3 genes were down-regulated. Conclusion:The effect of TRN treatment on hepatocarcinoma cell SMMC-7721 can be achieved through alteration of multiple genes expression,which provides important clues to understand the molecular mechanism of TRN effects on hepatocarcinoma cells.

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