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当前位置: 首页 > 文章 > 兔豆状囊尾蚴半胱氨酸蛋白酶基因的表达及免疫保护性分析 河南农业科学 2014,43 (1) 136-139+148
Position: Home > Articles > Cloning,Expression and Analysis of the Iimmunoprotection Activity of the Cysteine Protease fromTaenia pisiformis Journal of Henan Agricultural Sciences 2014,43 (1) 136-139+148

兔豆状囊尾蚴半胱氨酸蛋白酶基因的表达及免疫保护性分析

作  者：
王秋霞;马景周;张少华;骆学农;才学鹏
单  位：
河南科技学院动物科学学院;中国农业科学院兰州兽医研究所;方城县机电信息中等职业学校
关键词：
兔;豆状囊尾蚴;半胱氨酸蛋白酶;表达;重组蛋白;免疫保护试验
摘  要：
为探讨豆状囊尾蚴半胱氨酸蛋白酶的免疫保护性能,在毕赤酵母中表达半胱氨酸蛋白酶基因,表达产物经分子筛纯化后免疫试验家兔,3免之后进行人工攻虫保护试验,且以豆状囊尾蚴烯醇酶为对照进行同步免疫和攻虫试验。结果显示,成功表达了半胱氨酸蛋白酶蛋白,且该蛋白能够识别兔豆状囊尾蚴病阳性血清;半胱氨酸蛋白酶蛋白和豆状囊尾蚴烯醇酶抑虫效果均较差。结果表明,酶蛋白在免疫保护方面的功能比较弱,不适于做疫苗的候选抗原。
译  名：
Cloning,Expression and Analysis of the Iimmunoprotection Activity of the Cysteine Protease fromTaenia pisiformis
作  者：
WANG Qiu-xia;MA Jing-zhou;ZHANG Shao-hua;LUO Xue-nong;CAI Xue-peng;College of Animal Science,Henan Institute of Science and Technology;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;Fangcheng Mechanical and Electronic Information Secondary Vocational School;
关键词：
rabbit;;Cysticercosis pisiformis;;cysteine protease;;expression;;recombinant protein;;immune protection test
摘  要：
To investigate the immunogenicity of cathepsin L-like cysteine protease(TpCP),the TpCPgene sequence was cloned from T.pisiformis metacestodes and expressed in yeast.Western blot analysis showed that the recombinant protein could be recognized by the positive sera of rabbit against the whole Cysticercus pisiformis.Rabbits were immunized with the expressed protein that was purified by molecular sieve and feed eggs after determining antibody titer.The enolase of Cysticercus pisiformis was selected for further evaluating the possibility of zymoprotein as vaccine.The results showed that the immunoprotections induced by these two proteins were weak and unfit for vaccine preparation.

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