中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 茄子SmNAC1基因的克隆与表达分析 园艺学报 2014,41 (5) 975-984
Position: Home > Articles > Cloning and Expression Analysis of SmNAC1 in Solanum melongena Acta Horticulturae Sinica 2014,41 (5) 975-984

茄子SmNAC1基因的克隆与表达分析

作  者：
邵帅;徐岭贤;王绍辉;赵福宽
单  位：
北京农学院植物科学技术学院;北京农学院生物科学与工程学院
关键词：
茄子;NAC;克隆;基因表达;低温;高盐;赤霉素
摘  要：
以茄子幼苗为试材,利用cDNA末端快速扩增(RACE)技术获得SmNAC1基因全长,并利用RT-qPCR技术检测该基因在GA、4℃低温、NaCl处理下的时空表达。结果显示:SmNAC1全长序列1 279 bp,开放阅读框为909 bp,编码302个氨基酸,推测其蛋白质分子量约35.04 kD;SmNAC1含有NAC家族的N端保守域,与番茄、马铃薯同源性高达90%。SmNAC1受GA、低温和高盐诱导,表达上调,但在根、茎、叶中的表达量有差异,在根中表达水平整体高于茎和叶中,显示SmNAC1在根中优势表达,且短时间(0.5～1 h)相对表达量较高,而在茎和叶中应答相对迟缓,且叶中表达水平趋于稳定。可见SmNAC1可能参与了茄子对外源GA诱导的响应及对低温、高盐胁迫的耐受调节过程。
译  名：
Cloning and Expression Analysis of SmNAC1 in Solanum melongena
作  者：
SHAO Shuai;XU Ling-xian;WANG Shao-hui;ZHAO Fu-kuan;College of Biological Science and Engineering,Beijing University of Agriculture;College of Plant Science and Technology,Beijing University of Agriculture;
关键词：
eggplant;;NAC;;cloning;;gene express;;low temperature;;high salt;;GA
摘  要：
Eggplant seedlings were used as experimental materials and the full-length of SmNAC1 was cloned using Rapid-amplification of cDNA ends(RACE).The results showed that the full-length sequence of SmNAC1 is 1 279 bp and the open reading frame is 909 bp which encoding a polypeptide of 302 amino acids with a protein molecular weight of about 35.04 kD.SmNAC1 contains the N-terminal conserved domain of NAC family and homology was as high as 90% with tomato and potato.The expression patterns were analyzed by Real-time quantitative PCR(RT-qPCR)under GA,4 ℃ and NaCl treatment.The results showed that SmNAC1 was induced by GA and stress such as low temperature and high salt treatment.However,there were some differences of SmNAC1 gene expression in roots,stems and leaves.The overall level of expression in the roots was higher than stems and leaves under different treatments,which indicated that SmNAC1 was predominantly expressed in root.The relative expression level of SmNAC1 reached a high level with short time(0.5–1 h),while the reaction was relatively slow in stems and leaves.The relative expression level had the smallest variations in leaves under different treat times.The results suggested SmNAC1 may participate response of GA-induced and adjustment of low temperature and high salt stress tolerance in eggplant.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46