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当前位置: 首页 > 文章 > 水稻根系细胞膜H~+-ATPase对缺磷的反应 植物营养与肥料学报 2008,14 (2) 221-226
Position: Home > Articles > Response of plasma membrane H~+-ATPase of rice roots to the P deficiency Journal of Plant Nutrition and Fertilizers 2008,14 (2) 221-226

水稻根系细胞膜H~+-ATPase对缺磷的反应

作  者：
许征宇;狄廷均;朱毅勇;沈其荣;徐国华
单  位：
南京农业大学资源与环境科学学院
关键词：
水稻;细胞膜H+-ATPase;缺磷
摘  要：
用两相法分离了供磷(+P)和缺磷(-P)营养下水稻苗期根系的细胞膜,并测定了细胞膜上H+-ATPase的水解活性,以期阐明水稻根系细胞质膜上H+-ATPase对不同缺磷的反应机制。结果表明,缺磷的水稻根系细胞膜H+-ATPase的水解活性和H+-ATPase的Vmax,Km均低于正常供磷的植物;缺磷的水稻根系细胞膜H+-ATPase最佳pH值为6.0,而正常供磷植物的为pH 6.4左右;Western Blot结果说明,缺磷水稻根系细胞膜H+-ATPase酶浓度与正常供磷植物相似。本试验结果还说明,缺磷水稻根系细胞膜H+-ATPase活性低的原因并不是因为其单位细胞膜上的H+-ATPase酶分子数量小于正常供磷的植物,而是缺磷水稻根系细胞膜上H+-ATPase的同工酶的组成与供磷植物相比发生了变化。这很可能是缺磷胁迫下水稻根系细胞膜H+-ATPase的一种适应机制。
译  名：
Response of plasma membrane H~+-ATPase of rice roots to the P deficiency
作  者：
XU Zheng-yu,DI Ting-jun,ZHU Yi-yong,SHEN Qi-rong,XU Guo-hua (College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China)
关键词：
rice;plasma membrane H+-ATPase;P deficiency
摘  要：
Rice plants(Oryza sativa L.Japonica ssp.cv.Wuyujing) were fed with and without phosphate in hydroponics culture experiment.At the seedling stage the plasma membrane vesicles of roots were isolated by two-phase system.The plasma membrane H+-ATPase hydrolytic activity was analyzed for elucidating the response of the plasma membrane H+-ATPase of rice root to P deficiency.The results showed that:(1) plasma membrane H+-ATPase hydrolytic activity and ATPase K_m,Vmax obtained from P deficient rice root were lower than those from P sufficient rice root;(2) the optimum pH of the plasma membrane H+-ATPase of P deficient rice root was 6.2,but was 6.4 for that of P-sufficient root.(3) But the western blot showed that the enzyme concentration of plasma membrane H+-ATPase from P deficient rice root was similar to that from P sufficient rice root.The results indicated that the lower activity of H+-ATPase from P deficient rice root was not due to the decreased number of H+-ATPase units per membrane area.The different H+-ATPase isoforms might be induced in the P deficient rice root in comparison with the P sufficient rice root.It could be an adaptation mechanism of plasma membrane H+-ATPase of rice root to P deficiency.

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