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Position: Home > Articles > Detection of Bovine Viral Diarrhea Virus Based on Graphene Electrochemical Immunosensor Progress in Veterinary Medicine 2019 (12) 35-38

基于石墨烯电化学免疫传感器检测牛病毒性腹泻病毒

作  者:
黄娇玲;谢芝勋;范晴;谢志勤;谢丽基;邓显文;罗思思;曾婷婷;张艳芳;王盛;张民秀;刘加波;庞耀珊
单  位:
广西壮族自治区兽医研究所广西兽医生物技术重点实验室
关键词:
石墨烯;金纳米粒子;电化学免疫传感器;牛病毒性腹泻病毒
摘  要:
构建一种新型电化学免疫传感器用于检测牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV),为BVDV提供一种新型快速的检测方法。利用甲壳胺(Chi)修饰石墨烯(G)并负载金纳米粒子(AuNP),得到石墨烯-甲壳胺-金纳米粒子(G-Chi-AuNP)纳米复合物用于修饰金电极,然后固定抗BVDV NS3蛋白单克隆抗体,构建BVDV电化学免疫传感器。所构建的电化学免疫传感器对BVDV的检测敏感度为10~(1.2) TCID_(50),特异性试验结果表明,该方法对牛轮状病毒(BRV)、牛传染性鼻气管炎病毒(IBRV)、牛分支杆菌(MB)、牛冠状病毒(BCV)和猪瘟病毒(CSFV)无交叉反应。研究建立的BVDV电化学免疫传感器具有特异性好和灵敏度高的优点,在BVDV快速检测领域具有良好的应用前景。
译  名:
Detection of Bovine Viral Diarrhea Virus Based on Graphene Electrochemical Immunosensor
作  者:
HUANG Jiao-ling;XIE Zhi-xun;FAN Qing;XIE Zhi-qin;XIE Li-ji;DENG Xian-wen;LUO Si-si;ZENG Ting-ting;ZHANG Yan-fang;WANG Sheng;ZHANG Min-xiu;LIU Jia-bo;PANG Yao-shan;Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Vaterinary Biotechnology;
关键词:
graphene;;gold nanoparticles;;electrochemical immunoassay;;Bovine viral diarrhea virus
摘  要:
A novel electrochemical immunosensor was developed for sensitive detection of bovine viral diarrhea virus(BVDV).The aurum nanoparticles(AuNP) supported on chitosan(Chi) functionalized graphene(G) were used to carry monoclonal antibodies of bovine viral diarrhea virus,and the bovine viral diarrhea virus electrochemical immunosensor were constructed.The electrochemical immunosensor developed can detect the target bovine viral diarrhea virus at a titer higher than 6.7×10~2 TCID_(50).The developed electrochemical immunosensor was confirmed to be highly specific to 6.7×10~2 TCID_(50),and no positive results were observed when other pathogens were detected.This electrochemical immunosensor has a good specificity and high sensitivity,and it is promising for rapid detection of bovine viral diarrhea virus.

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