Voltammetric determination of nitrofurazone and its metabolite semicarbazide

ZHANG Xu-zhi;GU Xiao-lei;CHEN Bi-juan;MA Shao-sai;QU Ke-ming;ZHAO Chang-zhi;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;College of Chemistry & Molecular Engineering,Qingdao University of Science & Technology;

Graphene-based electrode;;Nitrofurazone;;Semicarbazide;;Voltammetry;;Fishery environmental monitoring

Voltammetric method for determining nitrofurazone and its metabolite semicarbazide was developed by employing grapheme-based electrode as working electrode. When the scan rate was 0. 10 V / s,an irreversible adsorption-controlled redox reaction of nitrofurazone occurred in the NaAcHAc buffer of pH 6. 0. Under the conditions of optimized parameters,including buffer solution,pH, accumulation time,etc,the dependence of the ipcon the concentration of nitrofurazone in the range of 20. 0- 800. 0 nmol / L was linear with a detection limit of 12. 0 nmol / L( S / N = 3). At the same scan rate,semicarbazide also had an irreversible adsorption-controlled redox reaction in the NaAc-HAc buffer of pH 4. 1. Under optimized conditions,linear relationship between ipaand the concentration ofsemicarbazide was obtained in the range of 1. 3- 39. 9 μmol / L with a detection limit of 0. 2 μmol / L. Under the other set of optimized parameters,nitrofurazone and semicarbazide could be detected in the same buffer solution subsequently,with the detection limit of 71. 0 nmol / L and 0. 3 μmol / L,respectively,and the average recovery was found to be 87% and 83%,respectively. The concentrations of the two molecules in real fishery water samples were successfully determined using this method.