Positive identification and differential expression of F1 generation of genetically modified pigs with the double genes of pGH and IGF-1

SONG Yuqin;FAN Yanyan;LI Xiaoling;WANG Yousheng;CUI Xiaorong;ZHANG Tingrong;SUN Jinhai;College of Animal Science and Technology,Qingdao Agricultural University;

genetically modified pig with double genes;;pGH gene;;IGF-1 gene;;positive identification;;fluorescence quantitative PCR

To explore the positive rate and expression differences of the F1 generation of genetically modified pigs with the double genes of p GH and IGF- 1,the methods of PCR detection,sequencing and fluorescence quantitative PCR were used to detect the mRNA expression levels of genetically modified pigs with the double genes of p GH and IGF- 1. The results showed that 17 positive pigs were found in 53 pigs of the F1 generation,and the positive rate was 32. 08%,which was confirmed that the p GH and IGF- 1 genes had been inherited to the F1 generation.There were no significant differences( P > 0. 05) in the average expression levels of the p GH and IGF- 1 genes between the genetically modified pigs and non- genetically modified pigs from one month to four months of age,but the average expression levels of p GH and IGF- 1 genes in the genetically modified pigs with the double genes were 1. 98 times and 2. 00 times higher than those in the non- genetically modified pigs at five months of age,respectively,while the difference were significant( P < 0. 05). The average expression levels of the p GH and IGF- 1 genes in the genetically modified pigs with the double genes were 1. 67 times and 1. 73 times higher than those in the non- genetically modified pigs at six month of age,respectively,while the differences were significant( P < 0. 05). The results indicate that the double genes of p GH and IGF- 1 have been inherited to the pigs of the F1 generation and can be stably expressed in pigs.