当前位置: 首页 > 文章 > 中国荷斯坦奶牛LOX-1基因3′UTR的PCR-SSCP分析 西北农林科技大学学报(自然科学版) 2007,35 (12) 16-20
Position: Home > Articles > Study on the 3′UTR of LOX-1 gene in Chinese Holstein by PCR-SSCP Journal of Northwest A & F University(Natural Science Edition) 2007,35 (12) 16-20

中国荷斯坦奶牛LOX-1基因3′UTR的PCR-SSCP分析

作  者:
邹荣婕;昝林森;姜向阳;张佳兰;申光磊
单  位:
西北农林科技大学动物科技学院
关键词:
中国荷斯坦奶牛;LOX-1基因;PCR-SSCP
摘  要:
为了寻找影响奶牛产奶性状的候选基因,采用PCR-SSCP分子标记方法,对中国荷斯坦奶牛LOX-1基因3′非编码区进行了多态性分析。结果表明,在中国荷斯坦奶牛中检测的LOX-1基因3′UTR有AA型、BB型和AB型3种基因型,其频率分别为0.1374,0.3130和0.5496;不同基因型与中国荷斯坦奶牛的乳脂率之间有显著的相关性(P<0.05),但对乳蛋白率、产奶量和体细胞评分(SCS)影响不大,未达到显著水平。初步认为LOX-1基因可能是控制中国荷斯坦奶牛产奶性能的一个候选基因,或是与之存在紧密遗传连锁的分子标记。
译  名:
Study on the 3′UTR of LOX-1 gene in Chinese Holstein by PCR-SSCP
作  者:
ZOU Rong-jie,ZAN Lin-sen,JIANG Xiang-yang,ZHANG Jia-lan,SHEN Guang-lei (College of Animal Sciences and Technology,Northwest A & F University,Yangling,Shaanxi 712100,China)
关键词:
Chinese Holstein;LOX-1 gene;PCR-SSCP
摘  要:
In order to detect polymorphisms in the lectin like oxidized low density lipoprotein receptor-1(LOX-1) gene,the polymorphism of LOX-1's 3′UTR in Chinese Holstein was detected through PCR-SSCP(Polymerase Chain Reaction-Single Strand Conformation Polymorphism).The result showed that three genotypes AA,BB and AB of the 3′UTR of LOX-1 gene were found,and the genotype frequency were 0.137 4,0.313 0,0.549 6,respectively in Chinese Holstein.There was highly significant correlation between different genotypes in fat content(P<0.05).No significant effect of different genotypes on their ME,protein content and SCS(Somatic Cell Score) was found.These results preliminarily showed that the LOX-1 gene was either a candidate gene that influenced the prolificacy in Chinese Holstein or a molecular marker in close linkage with such a gene.

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