In Situ Hybridization Analysis and Taxonomic Treatments of Rubus ellipticus,R. pinfaensis and R. ellipticus var. obcordatus

WANG Yan;WANG Xiao-rong;CHEN Qing;ZHANG Li;LIU Yin;TANG Hao-ru;Department of Horticulture,Sichuan Agricultural University;Institute of Pomology and Olericulture,Sichuan Agricultural University;

Rubus ellipticus;;Rubus pinfaensis;;Rubus ellipticus var.obcordatus;;fluorescence in situ hybridization(FISH);;genomic in situ hybridization(GISH);;taxonomic treatments

Chromosomal localization of 45S rDNA and 5S rDNA were studied by means of fluorescence in situ hybridization(FISH)on metaphase chromosomes of Rubus ellipticus,R.pinfaensis and R.ellipticus var.obcordatus.Genomic in situ hybridization(GISH)technique was also used to analyze the phylogenetic relationship among them.The results showed that two 45S rDNA and two 5S rDNA sites were detected in the three taxa.All 45S rDNA sites were localized on terminal chromosomal regions.5S rDNA loci localized on peri-centromeric region of short arms in R.ellipticus and R.pinfaensis,but located on terminal regions in R.ellipticus var.obcordatus.GISH results indicated that the genomes of R.ellipticus and R.pinfaensis shared many common DNA sequences on 14 chromosomes.Eight weak signals around centromere and telomere were shared between R.ellipticus and R.ellipticus var.obcordatus.Meanwhile,R.pinfanesis showed close relationship with R.parvifolius from subsection Stimulantes,distant with R.coreanus from subsection Pungentes.Combined the above data and morphological differences,we approved the view that R.ellipticus and R.pinfaensis should be combined and placed the combined species R.ellipticus into subsection Stimulantes.R.ellipticus var.obcordatus should be treated as R.obcordatus rather than as a variety of R.ellipticus.