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Position: Home > Articles > Study on Aqueous Two-phase Extraction of Flavonoids from Melaleuca ahemifolia Anhui Agricultural Science Bulletin 2014 (22) 39-41+64

双水相法提取澳洲茶树黄酮研究

作  者:
毕荣璐;王加兴;凌清华;熊智;刘祥义
单  位:
南森源化工有限公司;西南林业大学
关键词:
澳洲茶树;黄酮;提取;双水相法
摘  要:
为优化双水相法提取澳洲茶树中黄酮类化合物的条件,以澳洲茶树叶的总黄酮提取率为评价指标,分别对双水相的溶剂饱和程度、乙醇浓度、料液比、提取时间、提取温度对黄酮提取率影响进行了探究。结果表明,双水相提取优化条件为溶剂饱和度磷酸二氢钾∶乙醇1∶2(w/v),70%(v/v)乙醇,料液比1∶20(w/v),提取时间为90min,提取温度90℃。提取率最高6.74%。双水相提取澳洲茶树黄酮提取率较高,且分相时间短,不存在有机溶剂残留,易于工艺放大和连续操作,是一种理想的提取方法。
译  名:
Study on Aqueous Two-phase Extraction of Flavonoids from Melaleuca ahemifolia
作  者:
Bi Ronglu;Southwest Forestry University;
关键词:
Melaleuca ahemifolia;;Flavonoids;;Extraction;;Aqueous two-phase
摘  要:
The study was based on the evaluation index of flavonoids from melaleuca ahemifolia Extractionefficiency,to optimizate method of Aqueous two-phase extraction of flavonoid compounds in Australia teatree. The main research content including solvent saturated degree,ethanol concentration,solid-liquid ratio,extraction time,extraction temperature effect on flavonoids extraction yield of aqueous two-phase. The resultshowed that the conditions were ethanol 1∶2(w/v),70%(v/v)ethanol,material liquid than 1∶20(w/v),the ex-traction time was 90 min,extraction temperature 90℃,extraction efficiency rached to 6.74%. The rate of fla-vonoids is high by the method of aqueous two-phase,and the phase separation time is short,there is no or-ganic solvent residue,easy to process scale and continuous operation,is an ideal extraction method.

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