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当前位置: 首页 > 文章 > 黄瓜核心种质幼苗耐低温性评价及GWAS分析 植物遗传资源学报 2019 (6) 1606-1612
Position: Home > Articles > 黄瓜核心种质幼苗耐低温性评价及GWAS分析 Journal of Plant Genetic Resources 2019 (6) 1606-1612

黄瓜核心种质幼苗耐低温性评价及GWAS分析

作  者：
王伟平;宋子超;薄凯亮;董邵云;魏爽;苗晗;李锦斌;张圣平;顾兴芳
单  位：
中国农业科学院蔬菜花卉研究所
关键词：
黄瓜;核心种质;苗期;耐低温;GWAS
摘  要：
本研究以黄瓜核心种质为材料开展苗期耐低温鉴定和材料筛选,并进行全基因组关联分析,挖掘候选基因.在苗期(2叶1心时期),分2批播种,进行自然低温胁迫,2次处理平均温度分别为12℃和19.3℃,分别处理14 d和11 d,根据子叶和真叶的黄化症状进行分级和分组.处理后幼苗差异显著,两批次苗期调查变异系数分别为23.2%和31.7%.将供试核心种质划分为4个组,从87份核心种质材料中筛选出苗期耐低温材料CG45、CG61、CG88和CG104等.对苗期的低温鉴定数据,利用核心种质重测序信息进行GWAS分析,在Chr.1、Chr.3、Chr.4和Chr.5上分别检测到苗期耐低温位点gLTS1.1、gLTS3.1、gLTS4.1和gLTS5.1.其中,位点gLTS5.1对低温敏感,可以被重复检测到.本研究结果对耐低温黄瓜种质的选育及耐低温后续基因挖掘及功能验证具有一定的参考意义.
作  者：
WANG Wei-ping;SONG Zi-chao;BO Kai-liang;DONG Shao-yun;WEI Shuang;MIAO Han;LI Jin-bin;ZHANG Sheng-ping;GU Xing-fang;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;
关键词：
cucumber;;core germplasm;;seedling;;low temperature;;GWAS
摘  要：
In this study,the core collection of cucumber germplasm accessions were used to evaluate lowtemperature tolerance at seedling stage,and the candidate genes were identified via genome-wide association analysis.Two-mature-leaf seedling plants were exposed under low temperature conditions(12 ℃ or 19.3 ℃)for 14 days and 11 days,respectively.The classification of low-temperature tolerance was conducted based on the chilling injury index(CII)of cotyledons and true leaves.After treatments,the difference of seedlings was significant,and the coefficient of variation under two environmental conditions were 23.2% and 31.7%, respectively.These accessions were divided into four groups according to CII.Four accessions(CG45,CG61,CG88 and CG104)were shown to be tolerant under lowtemperature stress.The genome-wide association mapping of low temperature tolerance identified four loci including g LTS1.1,g LTS3.1,g LTS4.1 and g LTS5.1 that were assigned to the chromosomes 1,3,4 and 5 respectively.Among them,the locus gLTS5.1 can be detected repeatedly.Thus,this work provided insight for identifying low-temperature tolerant cucumber germplasm and unlocking the functional genes in future.

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