当前位置: 首页 > 文章 > 苜蓿雄性不育系组织培养技术的研究 中国草地学报 2006,28 (5) 29-32
Position: Home > Articles > Studies on Tissue Culture Techniques of Male Sterile Line of Alfalfa Chinese Journal of Grassland 2006,28 (5) 29-32

苜蓿雄性不育系组织培养技术的研究

作  者:
高翠萍;石凤翎;孙静
单  位:
内蒙古农业大学生态环境学院
关键词:
苜蓿;雄性不育系;组织培养;体细胞胚
摘  要:
以苜蓿雄性不育系Ms-4幼嫩茎段、叶片、叶柄为外植体,诱导苜蓿雄性不育系再生植株。结果表明:叶柄是诱导愈伤组织的良好外植体,体细胞胚诱导率为90%,再生率49%。苜蓿雄性不育系插条生根的最佳处理为接种前用水进行预处理0.5h,外植体为完整插条,培养基为A1、A2,其生根率为97%,生根系数为5.3。
译  名:
Studies on Tissue Culture Techniques of Male Sterile Line of Alfalfa
作  者:
GAO Cui-ping,SHI Feng-ling,SUN Jing(College of Ecology and Environmental Science,Inner Mongolia Agricultural University,Hohhot 010019,China)
关键词:
Alfalfa;Male sterile line;Tissue culture;Somatic embryos
摘  要:
The infancy stem segments,leaves and leafstalks of male sterile line Ms-4 of Medicago varia Martin.cv.Caoyuan No.1 were taken as explants and induced somatic embryos and roots of male sterile line Ms-4 of Medicago varia Martin.cv Caoyuan No.1.The results showed that leafstalks are good explants for inducing callus.Somatic embryos inducing rates was 90%,regeration rate was 49%.The best treatment of rooting of quickset of male sterile line Ms-4 of Medicago varia Martin.cv.Caoyuan No.1 is water treat for 0.5h before inoculabitity,integrity quickset,and A1 and A2 rooting medium,rooting rates was 97%,rooting coefficient was 5.3.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊