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当前位置: 首页 > 文章 > DEB-2菌株对贵州苹果早期落叶病病原的毒力效应 西南农业学报 2014,27 (2) 652-657
Position: Home > Articles > Toxicity of Endophyte DEB-2 to Pathogens of Guizhou Apple Early Defoliation Disease Southwest China Journal of Agricultural Sciences 2014,27 (2) 652-657

DEB-2菌株对贵州苹果早期落叶病病原的毒力效应

作  者：
何劲;雷帮星;宋贞富;乔光;宋莎;康冀川;文晓鹏
单  位：
贵州省果树科学研究所;贵州省农业生物工程重点实验室;贵阳学院;贵州大学生命科学学院;西南药用生物资源教育部工程研究中心
关键词：
苹果早期落叶病;苹果轮斑病;苹果斑点落叶病;DEB-2菌株;毒力
摘  要：
为苹果早期落叶病的生物防治提供依据,采用组织分离法分离病原、形态与分子鉴定方法鉴定贵州苹果落叶病病原,用生物测定法研究DEB-2菌株对贵州苹果早期落叶病病原的毒力效应。结果表明:贵州苹果早期落叶病主要为苹果轮斑病和苹果斑点落叶病,其病原分别为轮斑病病原(Alternaria mali)和苹果斑点落叶病病原(Altemaria alternata);DEB-2菌株发酵液对A.mali和A.alternata抑制菌丝生长的EC50值分别为1.34%和0.12%;对抑制孢子萌发的EC50值分别为2.32%和5.32%。DEB-2菌株发酵液有效地抑制了贵州苹果早期落叶病原菌的菌丝生长和孢子萌发。
译  名：
Toxicity of Endophyte DEB-2 to Pathogens of Guizhou Apple Early Defoliation Disease
作  者：
HE Jin;LEI Bang-xing;SONG Zheng-fu;QIAO Guang;SONG Sha;KANG Ji-chuan;WEN Xiao-peng;Guiyang University;The Research and Engineering Center for Southwest Bio-pharmaceutical Resources of National Education Ministry of China;College of Life Sciences,Guizhou University;Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University;Guizhou Institute of Fruit;
关键词：
Apple early defoliation disease;;Alternaria mali;;Altemaria alternata;;DEB-2;;Virulence
摘  要：
To provide a reference for biological control of apple early defoliation disease,pathogens were isolated using tissue separation method and identified by morphological and molecular methods. The virulence of endophyte DEB-2 against the pathogens was tested by bioassay method. The results showed that the pathogens of apple early defoliation disease in Guizhou were Alternaria alternata and Alternaria mali.The EC50values of DEB-2 fermented liquid inhibiting mycelial growth of A. alternata and A. mali were 1. 34 % and 0. 12 %,respectively.The EC50values of DEB-2 fermented liquid inhibiting spore germination of A. alternata and A. mali were 2. 32 % and 5. 32 %,respectively. Therefore,endophyteic strain DEB-2 could effectively inhibit the mycelial growth and spore germination.

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