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Position: Home > Articles > Isolation and Identification of Pathogenic Fungus of Botrytis cinerea and Screening of Antagonistic Bacteria Against Tomato Gray Mold Biotechnology Bulletin 2017 (8) 81-87

番茄灰霉病病原菌分离鉴定及拮抗菌筛选

作  者:
陈哲;黄静;赵佳;梁宏
单  位:
山西省农业科学院生物技术研究中心
关键词:
灰葡萄孢菌;解淀粉芽孢杆菌;巨大芽孢杆菌;生物防治
摘  要:
灰葡萄孢菌(Botrytis cinerea)引起的番茄灰霉病是番茄生产上的重要病害。从番茄果实表面成功分离了1株真菌BC2016-2,经过鉴定确定其为灰霉病的病原菌——灰葡萄孢菌;以灰葡萄孢菌BC2016-2为指示菌,筛选到了3株具有明显抑制作用的菌株,分别为解淀粉芽孢杆菌(Bacillus amyloliquefacien)CM3、巨大芽孢杆菌(Bacillus megaterium)Y-30和解淀粉芽孢杆菌Y-48。盆栽实验结果显示,菌株CM3、Y-30和Y-48对灰葡萄孢菌BC2016-2引起的番茄灰霉病的防治效果分别为65.58%、54.1%和72.13%。
译  名:
Isolation and Identification of Pathogenic Fungus of Botrytis cinerea and Screening of Antagonistic Bacteria Against Tomato Gray Mold
作  者:
CHEN Zhe;HUANG Jing;ZHAO Jia;LIANG Hong;Biotechnology Research Center,Shanxi Academy of Agriculture Science;
关键词:
Botrytis cinerea;;Bacillus amyloliquefaciens;;Bacillus megaterium;;biological control
摘  要:
It is well known that Botrytis cinerea is one of the most geographically widespread plant pathogen on tomato. A fungusBC2016-2 was successfully isolated from the tomato surface and identified as B. cinerea,a pathogen of tomato gray mold. Then,three strainswith obvious inhibitory effects were screened using B. cinerea BC2016-2 as indicator fungus,and they were Bacillus amyloliquefacien CM3,Bacillus megaterium Y-30 and Bacillus amyloliquefaciens Y-48. The greenhouse pot experiment showed that the control effects of CM3,Y-30 and Y-48 on the tomato gray mold caused by B. cinerea BC2016-2 were 65.58%,54.1% and 72.13%,respectively.

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